Zonguldak Bulent Ecevit University, Faculty of Medicine, Department of Histology and Embryology, Zonguldak, Turkey.
Zonguldak Bulent Ecevit University Faculty of Medicine, Department of Medical Biology, Zonguldak, Turkey.
Biomed Pharmacother. 2020 Jul;127:110134. doi: 10.1016/j.biopha.2020.110134. Epub 2020 Apr 30.
This study aimed to explore whether PARP-1 regulatory pathway mediated X irradiation induced cell cycle arrest and apoptosis or not. In this regard, colonic mucosal injury caused by whole-body X-irradiation induced apoptosis through PARP-1, caspase 3 and p53 regulatory pathway were evaluated in experimental rat models. Eighteen Wistar albino rats were divided into three groups. Two radiation groups received 8.3 Gy dose of whole-body X-irradiation as a single dose and the control group received physiological saline intraperitoneally. Radiation groups were sacrificed after 6 h and 4 days of irradiation. PARP-1 and caspase 3 expression in the nuclei of colonic crypt cells significantly increased 6 h after irradiation, and declined 4 days after irradiation. In conflict with other studies that reported p53 as not being expressed widely in colonic mucosa, in our study the expressions of p53 were elevated both in the cytoplasm and in the nucleus of the crypt cells, especially 6 h after irradiation. In the radiation groups, colonic mucosal injury score was significantly elevated compared with that of the control group. Our data demonstrated that PARP-1, caspase-3 and p53 expression increased in colonic mucosa 6 h after irradiation.
本研究旨在探讨聚腺苷二磷酸核糖聚合酶-1（PARP-1）调控通路是否介导 X 射线照射诱导的细胞周期停滞和细胞凋亡。在这方面，通过 PARP-1、半胱天冬酶 3 和 p53 调控通路，评估了全身 X 射线照射引起的结肠黏膜损伤诱导的细胞凋亡，实验大鼠模型中。将 18 只 Wistar 白化大鼠分为三组。两组照射组接受 8.3Gy 全身 X 射线单次照射，对照组给予腹腔内生理盐水。照射组在照射后 6 小时和 4 天处死。照射后 6 小时，结肠隐窝细胞核中 PARP-1 和半胱天冬酶 3 的表达明显增加，4 天后下降。与其他报道 p53 在结肠黏膜中不广泛表达的研究结果相矛盾，在我们的研究中，p53 的表达在隐窝细胞的细胞质和细胞核中均升高，尤其是在照射后 6 小时。在照射组中，与对照组相比，结肠黏膜损伤评分显著升高。我们的数据表明，PARP-1、半胱天冬酶 3 和 p53 的表达在照射后 6 小时增加。
