el-Borai M H, Taylor P V, Norman A, Gowland G, Hancock K W, Scott J S
Department of Obstetrics and Gynaecology, General Infirmary, University of Leeds, Belmont Grove, U.K.
J Reprod Immunol. 1988 Nov;14(2):115-23. doi: 10.1016/0165-0378(88)90063-0.
In 36 women with unexplained primary recurrent abortion, 13 with secondary unexpained recurrent abortion, 25 with primary unexplained infertility, 7 with secondary unexplained infertility and two groups of control women, autoantibodies to soluble cellular antigens were measured by Western blotting to a disaggregated HeLa cell antigen preparation, by counter immunoelectrophoresis and by indirect immunofluorescence. Using Western blotting the women with primary infertility and those with secondary recurrent abortion had a significantly higher prevalence of autoantibodies (P less than 0.01 in each case). This was not shown using the other methods. It is possible that these antibodies could be causally related to the pathology of the conditions studied.
对36例不明原因的原发性复发性流产女性、13例继发性不明原因复发性流产女性、25例原发性不明原因不孕症女性、7例继发性不明原因不孕症女性以及两组对照女性，采用蛋白质印迹法检测针对经分散的HeLa细胞抗原制剂的可溶性细胞抗原自身抗体，采用对流免疫电泳法和间接免疫荧光法进行检测。通过蛋白质印迹法发现，原发性不孕症女性和继发性复发性流产女性的自身抗体患病率显著更高（每种情况P均小于0.01）。采用其他方法未显示出这一结果。这些抗体有可能与所研究疾病的病理过程存在因果关系。
