Shastri Padmaja, Green Judy, Matias Fernando, Kalmokoff Martin L, Green-Johnson Julia M, Brooks Stephen P J
Applied Bioscience Graduate Program and Faculty of Science, University of Ontario Institute of Technology, Oshawa, Ontario L1H 7K4, Canada.
Bureau of Nutritional Sciences, Health Canada, Ottawa, Ontario K1A 0K9, Canada.
J Funct Foods. 2017 Dec;39:306-311. doi: 10.1016/j.jff.2017.10.021. Epub 2017 Nov 2.
Immune measures and the fecal bacterial community were examined in female Biobreeding rats housed in wire bottom cages (wire) or in solid bottom cages containing hardwood chips (bedding). Housing did not affect food intake, weight gain, fecal output or fibre content, serum liver enzymes, or spleen and mesenteric lymph node immune cell populations. Bedding-housed rat feces were enriched in phylotypes aligning within the phylum (families and ) and had a 2-fold lower content of phylotypes aligning within the phylum . Feces from bedding-housed rats also contained significantly more acetic acid and less propionic, isobutyric, valeric and isovaleric acids than those housed on wire. Bedding-housed rats had significantly higher splenic concentrations of interleukin-4 ( < 0.001). These results demonstrate that bedding can indirectly influence systemic and mucosal immune measures, potentially adding additional complexities and confounding results to nutrition studies investigating the health effects of dietary fibres.
对饲养在金属丝底笼（金属丝笼）或装有硬木屑的实底笼（铺垫物笼）中的雌性BioBreeding大鼠的免疫指标和粪便细菌群落进行了检测。饲养方式不影响食物摄入量、体重增加、粪便排出量或纤维含量、血清肝酶，或脾脏和肠系膜淋巴结免疫细胞群体。饲养在铺垫物笼中的大鼠粪便中，属于某一门（某两个科）的系统发育型富集，而属于另一门的系统发育型含量则低2倍。与饲养在金属丝笼中的大鼠相比，饲养在铺垫物笼中的大鼠粪便中乙酸含量也显著更高，而丙酸、异丁酸、戊酸和异戊酸含量更低。饲养在铺垫物笼中的大鼠脾脏中白细胞介素-4浓度显著更高（P<0.001）。这些结果表明，铺垫物可间接影响全身和黏膜免疫指标，这可能会给研究膳食纤维对健康影响的营养研究增加额外的复杂性并混淆结果。
