Department of Plant Protection, College of Agriculture, University of Zanjan, 45371-38791Zanjan, Iran.
Department of Plant Protection, School of Agriculture, University of Shiraz, 71441-65186Shiraz, Iran.
J Helminthol. 2020 May 4;94:e149. doi: 10.1017/S0022149X20000279.
Thada populus n. sp. was found in the rhizosphere of Populus euphratica in the city of Dezful, south-western Iran. The new species is characterized by its 365-453 μm body length, cuticle with transverse annuli 0.9-1.2 μm wide, lateral fields with four lines, lip region low with one or two annuli, 7.0-8.5 μm wide at base, longitudinal or slightly sigmoid amphidial slit, delicate stylet 8.4-9.8 μm long with posteriorly directed knobs, dorsal pharyngeal gland opening at 1.0-1.5 μm posterior to the stylet knobs, non-muscular and valve-less median bulb, pyriform and offset basal bulb, almost oval spermatheca, posterior position of vulva (V = 75-79%) without lateral membrane, short post-vulval uterine sac and conical tail with finely to broadly rounded terminus. Morphological differences of the new species with those of the type species, Thada striata, are discussed. Molecular phylogenetic studies of the new species using partial sequences of small subunit ribosomal DNA revealed that the new species formed a clade with Malenchus spp., Filenchus facultativus, F. fungivorus and Filenchus sp. in Bayesian inference. Morphological and molecular phylogenetic studies were also performed on Tenunemellus indicus, the second recovered species, the latter analysis using partial sequences of large subunit ribosomal DNA. In the inferred Bayesian tree, T. indicus formed a clade with Labrys fuzhouensis, Lelenchus leptosoma from the Netherlands, Malenchus spp. and Filenchus discrepans.
在伊朗西南部城市德黑兰，发现了一种新的白杨根际 Thada populus n. sp.。该新种的特征为体长 365-453μm，具横带纹的角质层宽 0.9-1.2μm，侧带具 4 条线，唇区低，具 1-2 个环纹，基部宽 7.0-8.5μm，纵或稍“S”形的口孔缝，细长的口针 8.4-9.8μm，后端具指向后的棘，口针后区具一对后端指向的棘，无肌质、无瓣中食道腺，瓶形且偏位的基球囊，近梨形、偏位的交合囊，后阴门（V = 75-79%）无侧膜，阴门后很短的子宫囊和具细至宽圆端的锥形尾。新种与模式种 Thada striata 的形态差异进行了讨论。使用小亚基核糖体 DNA 部分序列对新种进行分子系统发育研究，结果表明新种与 Malenchus spp.、Filenchus facultativus、F. fungivorus 和 Filenchus sp. 在贝叶斯推断中形成一个分支。对第二个回收物种 Tenunemellus indicus 也进行了形态学和分子系统发育研究，后者使用大亚基核糖体 DNA 部分序列进行分析。在推断的贝叶斯树中，T. indicus 与来自中国福州的 Labrys fuzhouensis、荷兰的 Lelenchus leptosoma、Malenchus spp. 和 Filenchus discrepans 形成一个分支。
