Rodrigues Emille M, Rutajoga Nelson, Rioux David, Yvon-Leroux Jacob, Hemmer Eva
Department of Chemistry and Biomolecular Sciences, University of Ottawa.
Photon etc.
J Vis Exp. 2020 Apr 14(158). doi: 10.3791/60826.
In this work, we describe a protocol for a novel application of hyperspectral imaging (HSI) in the analysis of luminescent lanthanide (Ln)-based molecular single crystals. As representative example, we chose a single crystal of the heterodinuclear Ln-based complex [TbEu(bpm)(tfaa)6] (bpm=2,2'-bipyrimidine, tfaa =1,1,1-trifluoroacetylacetonate) exhibiting bright visible emission under UV excitation. HSI is an emerging technique that combines 2-dimensional spatial imaging of a luminescent structure with spectral information from each pixel of the obtained image. Specifically, HSI on single crystals of the [Tb-Eu] complex provided local spectral information unveiling variation of the luminescence intensity at different points along the studied crystals. These changes were attributed to the optical anisotropy present in the crystal, which results from the different molecular packing of Ln ions in each one of the directions of the crystal structure. The HSI herein described is an example of the suitability of such technique for spectro-spatial investigations of molecular materials. Yet, importantly, this protocol can be easily extended for other types of luminescent materials (such as micron-sized molecular crystals, inorganic microparticles, nanoparticles in biological tissues, or labelled cells, among others), opening many possibilities for deeper investigation of structure-property relationships. Ultimately, such investigations will provide knowledge to be leveraged into the engineering of advanced materials for a wide range of applications from bioimaging to technological applications, such as waveguides or optoelectronic devices.
在这项工作中，我们描述了一种用于高光谱成像（HSI）在基于发光镧系（Ln）的分子单晶分析中的新应用的方案。作为代表性示例，我们选择了异双核Ln基配合物[TbEu(bpm)(tfaa)6]（bpm = 2,2'-联嘧啶，tfaa = 1,1,1-三氟乙酰丙酮）的单晶，该单晶在紫外激发下表现出明亮的可见发射。HSI是一种新兴技术，它将发光结构的二维空间成像与所获得图像每个像素的光谱信息相结合。具体而言，对[Tb-Eu]配合物单晶进行的HSI提供了局部光谱信息，揭示了沿研究晶体不同点处发光强度的变化。这些变化归因于晶体中存在的光学各向异性，这是由晶体结构各个方向上Ln离子的不同分子堆积所导致的。本文所述的HSI是该技术适用于分子材料光谱空间研究的一个示例。然而，重要的是，该方案可以很容易地扩展到其他类型的发光材料（如微米级分子晶体、无机微粒、生物组织中的纳米颗粒或标记细胞等），为深入研究结构-性质关系开辟了许多可能性。最终，此类研究将提供知识，以用于从生物成像到技术应用（如波导或光电器件）等广泛应用的先进材料工程。
