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外源性麦芽糖增强斑马鱼对左氧氟沙星耐药哈维氏弧菌的免疫力。

Exogenous maltose enhances Zebrafish immunity to levofloxacin-resistant Vibrio alginolyticus.

机构信息

The Third Affiliated Hospital, State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.

出版信息

Microb Biotechnol. 2020 Jul;13(4):1213-1227. doi: 10.1111/1751-7915.13582. Epub 2020 May 4.

DOI:10.1111/1751-7915.13582
PMID:32364684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7264874/
Abstract

Understanding the interplay between bacterial fitness, antibiotic resistance, host immunity and host metabolism could guide treatment and improve immunity against antibiotic-resistant pathogens. The acquisition of levofloxacin (Lev) resistance affects the fitness of Vibrio alginolyticus in vitro and in vivo. Lev-resistant (Lev-R) V. alginolyticus exhibits slow growth, reduced pathogenicity and greater resistance to killing by the host, Danio rerio (zebrafish), than Lev-sensitive (Lev-S) V. alginolyticus, suggesting that Lev-R V. alginolyticus triggers a weaker innate immune response in D. rerio than Lev-S V. alginolyticus. Differences were detected in the metabolome of D. rerio infected with Lev-S or Lev-R V. alginolyticus. Maltose, a crucial metabolite, is significantly downregulated in D. rerio infected with Lev-R V. alginolyticus, and exogenous maltose enhances the immune response of D. rerio to Lev-R V. alginolyticus, leading to better clearance of the infection. Furthermore, we demonstrate that exogenous maltose stimulates the host production of lysozyme and its binding to Lev-R V. alginolyticus, which depends on bacterial membrane potential. We suggest that exogenous exposure to crucial metabolites could be an effective strategy for treating and/or managing infections with antibiotic-resistant bacteria.

摘要

理解细菌适应性、抗生素耐药性、宿主免疫和宿主代谢之间的相互作用，可以指导治疗并提高对抗生素耐药病原体的免疫力。左氧氟沙星（Lev）耐药性的获得会影响体外和体内 Alg 弧菌的适应性。与左氧氟沙星敏感（Lev-S）Alg 弧菌相比，左氧氟沙星耐药（Lev-R）Alg 弧菌的生长速度较慢，致病性降低，对宿主（斑马鱼）的杀伤作用更强，这表明与 Lev-S Alg 弧菌相比，Lev-R Alg 弧菌在斑马鱼中引发的先天免疫反应较弱。Lev-S 或 Lev-R Alg 弧菌感染的斑马鱼的代谢组学存在差异。麦芽糖是一种关键代谢物，在感染 Lev-R Alg 弧菌的斑马鱼中显著下调，外源性麦芽糖增强了斑马鱼对 Lev-R Alg 弧菌的免疫反应，从而更好地清除感染。此外，我们证明外源性麦芽糖刺激宿主产生溶菌酶并将其与 Lev-R Alg 弧菌结合，这取决于细菌膜电位。我们认为，外源性暴露于关键代谢物可能是治疗和/或管理抗生素耐药菌感染的有效策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/d4c5eede5b31/MBT2-13-1213-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/a0e1f972e290/MBT2-13-1213-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/d4c5eede5b31/MBT2-13-1213-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/918e5e1ba6ee/MBT2-13-1213-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/5809ef448b01/MBT2-13-1213-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/b2c24b8d4eda/MBT2-13-1213-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb88/7264874/d4c5eede5b31/MBT2-13-1213-g008.jpg

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