Electrochemistry Laboratory for Sensors & Energy (ELSE), Incheon National University, Incheon, 22012, Republic of Korea.
Electrochemistry Laboratory for Sensors & Energy (ELSE), Incheon National University, Incheon, 22012, Republic of Korea.
Biosens Bioelectron. 2020 Aug 1;161:112237. doi: 10.1016/j.bios.2020.112237. Epub 2020 Apr 28.
An optical and colorimetric biosensor comprising gold nanowires (Au NWs) templated with genetically engineered M13 bacteriophages expressing a specific Au binding peptides tyrosine-glutamic acid-glutamic acid-glutamic acid (Y3E) is fabricated by silver nitrate and surfactant-mediated biomineralization process. The diameter of the Y3E-Au NWs is around 10 nm and an oriented growth mechanism is identified for the continuous growth of the NWs by interconnecting M13 bacteriophages. The flexible Au NWs have formed an enriched Hg(II) binding sites on its surface and the surface-coated silver nanophase functions as a receptor for more efficient Hg(II) binding. Amalgamation-based colorimetric and optical Hg(II) biosensing of Au NWs are scrutinized in the presence of wild-type M13 bacteriophage-templated Au NWs and spherical Au nanoparticles. It is demonstrated that in comparison with the spherical Au nanoparticles, Y3E-Au NWs exhibits an aggregation-free optical and colorimetric sensor for Hg(II). Mechanistic investigation for the aggregation-free sensor and the Au-Hg amalgam crystals are carried out using TEM, STEM-EDX and XPS analyses.
一种基于金纳米线（Au NWs）的光学和比色生物传感器，由表达特定 Au 结合肽酪氨酸-谷氨酸-谷氨酸-谷氨酸（Y3E）的基因工程 M13 噬菌体模板制成，通过硝酸银和表面活性剂介导的生物矿化过程制备。Y3E-Au NWs 的直径约为 10nm，通过连接 M13 噬菌体确定了 NWs 的连续生长的定向生长机制。柔性 Au NWs 在其表面形成了丰富的 Hg(II)结合位点，表面涂覆的银纳米相作为更有效的 Hg(II)结合的受体。在野生型 M13 噬菌体模板化 Au NWs 和球形 Au 纳米颗粒的存在下，对基于共沉淀的 Au NWs 的比色和光学 Hg(II)生物传感器进行了研究。结果表明，与球形 Au 纳米颗粒相比，Y3E-Au NWs 表现出无聚集的光学和比色 Hg(II)传感器。使用 TEM、STEM-EDX 和 XPS 分析对无聚集传感器和 Au-Hg 共晶晶体进行了机理研究。
