Visani Michela, Marucci Gianluca, Biase Dario de, Giangaspero Felice, Buttarelli Francesca Romana, Brandes Alba Ariela, Franceschi Enrico, Acquaviva Giorgia, Ciarrocchi Alessia, Rhoden Kerry Jane, Tallini Giovanni, Pession Annalisa
Department of Specialized, Diagnostic and Experimental Medicine, Anatomic Pathology-Molecular Diagnostic Unit AUSL-IRCCS of Bologna, University of Bologna School of Medicine, 40138 Bologna, Italy.
Anatomic Pathology Unit, Ospedale Bellaria AUSL-IRCCS of Bologna, 40139 Bologna, Italy.
Diagnostics (Basel). 2020 Apr 29;10(5):265. doi: 10.3390/diagnostics10050265.
Medulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA expression between childhood and adult medulloblastoma. Medulloblastomas were profiled for microRNA expression using the Exiqon Human miRNome panel (I + II) analyzing 752 microRNAs in a training set of six adult and six childhood cases. Then, the most differentially expressed microRNAs were validated in a total of 21 adult and 19 childhood cases. Eight microRNAs (miR-196b-5p, miR-183-5p, miR-200b-3p, miR-196a-5p, miR-193a-3p, miR-29c-3p, miR-33b-5p, and miR-200a-3p) were differentially expressed in medulloblastoma of adults and children. Analysis of the validation set confirmed that miR-196b-5p and miR-200b-3p were significantly overexpressed in medulloblastoma of adults as compared with those of children. We followed an in silico approach to investigate direct targets and the pathways involved for the two microRNAs (miR-196b and miR-200b) differently expressed between adult and childhood medulloblastoma. Adult and childhood medulloblastoma have different miRNA expression profiles. In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies.
髓母细胞瘤是一种侵袭性很强的脑肿瘤，通常影响儿童，而在成人中它占所有脑肿瘤的约1%。关于罕见的成人髓母细胞瘤的微小RNA表达谱知之甚少。本研究的主要目的是确定儿童和成人髓母细胞瘤之间微小RNA表达的特殊差异。使用Exiqon人类miRNome panel（I + II）对髓母细胞瘤进行微小RNA表达分析，在一个包含6例成人和6例儿童病例的训练集中分析752种微小RNA。然后，在总共21例成人和19例儿童病例中对差异表达最明显的微小RNA进行验证。8种微小RNA（miR-196b-5p、miR-183-5p、miR-200b-3p、miR-196a-5p、miR-193a-3p、miR-29c-3p、miR-33b-5p和miR-200a-3p）在成人和儿童髓母细胞瘤中差异表达。对验证集的分析证实，与儿童髓母细胞瘤相比，miR-196b-5p和miR-200b-3p在成人髓母细胞瘤中显著过表达。我们采用计算机模拟方法研究在成人和儿童髓母细胞瘤中差异表达的两种微小RNA（miR-196b和miR-200b）的直接靶点和相关途径。成人和儿童髓母细胞瘤具有不同的微小RNA表达谱。特别是，miR-196b-5p和miR-200b-3p的差异失调是成人髓母细胞瘤微小RNA谱的特征，并为新的诊断、预后或治疗策略提供了潜在靶点。
