Majdi Chaimae, Pereira Carla, Dias Maria Inês, Calhelha Ricardo C, Alves Maria José, Rhourri-Frih Boutayna, Charrouf Zoubida, Barros Lillian, Amaral Joana S, Ferreira Isabel C F R
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
CBMN-UMR 5248, Faculty of Pharmacy, University of Bordeaux, 33016 Bordeaux, France.
Antioxidants (Basel). 2020 Apr 29;9(5):369. doi: 10.3390/antiox9050369.
The aim of this work was to contribute to the knowledge on the chemical composition and bioactive properties of two species of the genus, namely cultivar 'Cinammon' and . For this purpose, samples of these plants grown in Portugal were evaluated for their composition in phenolic and volatile compounds, and the infusion and hydroethanolic extracts were assessed for their in vitro antioxidant, antimicrobial, cytotoxic, and anti-inflammatory activities. In total, the two basil samples showed the presence of seven caffeic acid and derivatives (dimers, trimers, and tetramers) and five flavonoids, mainly glycoside derivatives of quercetin. Despite some qualitative and quantitative differences, in both samples rosmarinic acid was the major phenolic compound, and linalool the predominant volatile compound. In general, the tested extracts provided relevant bioactive properties since both basil species showed higher antioxidant activity in Thiobarbituric Acid Reactive Substances (TBARs) and Oxidative Hemolysis Inhibition (OxHLIA) assays when compared with the positive control Trolox. Despite extracts showing slightly better activity against some strains, both types of extracts evidenced similar antimicrobial activity, being more active against Gram-positive bacteria. The extracts also revealed interesting cytotoxicity, particularly the hydroethanolic extract which was also the only one exhibiting anti-inflammatory activity.
这项工作的目的是增进对该属两个品种，即‘肉桂’品种和……化学成分及生物活性特性的了解。为此，对在葡萄牙种植的这些植物样本的酚类和挥发性化合物成分进行了评估，并对其浸剂和水乙醇提取物的体外抗氧化、抗菌、细胞毒性和抗炎活性进行了评估。总体而言，这两种罗勒样本显示含有七种咖啡酸及其衍生物（二聚体、三聚体和四聚体）和五种黄酮类化合物，主要是槲皮素的糖苷衍生物。尽管存在一些定性和定量差异，但在两个样本中，迷迭香酸都是主要的酚类化合物，芳樟醇是主要的挥发性化合物。一般来说，所测试的提取物具有相关的生物活性，因为与阳性对照曲克芦丁相比，这两种罗勒品种在硫代巴比妥酸反应物质（TBARs）和氧化溶血抑制（OxHLIA）试验中均表现出更高的抗氧化活性。尽管浸剂对某些菌株的活性略好，但两种类型的提取物抗菌活性相似，对革兰氏阳性菌更具活性。提取物还显示出有趣的细胞毒性，特别是水乙醇提取物，它也是唯一具有抗炎活性的提取物。
