Department of Medical Technology, Graduate School of Health Sciences, Okayama University, Okayama 700-8558, Japan.
Department of Molecular Biology and Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Int J Mol Sci. 2020 Apr 29;21(9):3140. doi: 10.3390/ijms21093140.
In patients with osteoarthritis (OA), there is a decrease in both the concentration and molecular size of hyaluronan (HA) in the synovial fluid and cartilage. Cell migration-inducing hyaluronidase 1 (CEMIP), also known as hyaluronan (HA)-binding protein involved in HA depolymerization (HYBID), was recently reported as an HA depolymerization-related molecule expressed in the cartilage of patients with OA. However, the underlying mechanism of CEMIP regulation is not well understood. We found that CEMIP expression was transiently increased by interleukine-1β (IL-1β) stimulation in chondrocytic cells. We also observed that ERK activation and NF-κB nuclear translocation were involved in the induction of CEMIP by IL-1β. In addition, both administration of HA and mechanical strain attenuated the CEMIP induction in IL-1β-stimulated chondrocytes. In conclusion, we clarified the regulatory mechanism of CEMIP in chondrocytes by inflammatory cytokines and suggested the potential involvement in osteoarthritis development.
在骨关节炎（OA）患者的滑液和软骨中，透明质酸（HA）的浓度和分子大小均降低。细胞迁移诱导透明质酸酶 1（CEMIP），也称为参与透明质酸解聚的透明质酸（HA）结合蛋白（HYBID），最近被报道为 OA 患者软骨中表达的与透明质酸解聚相关的分子。然而，CEMIP 调节的潜在机制尚不清楚。我们发现白细胞介素 1β（IL-1β）刺激可使软骨细胞中的 CEMIP 表达短暂增加。我们还观察到 ERK 激活和 NF-κB 核易位参与了 IL-1β诱导的 CEMIP。此外，HA 的给药和机械应变均减弱了 IL-1β刺激的软骨细胞中 CEMIP 的诱导。总之，我们通过炎症细胞因子阐明了软骨细胞中 CEMIP 的调节机制，并提示其可能参与骨关节炎的发生。
