Department of Emergency and Organ Transplantation (D.E.T.O.), Hematology Section, University of Bari, 70124 Bari, Italy.
Int J Mol Sci. 2020 Apr 29;21(9):3141. doi: 10.3390/ijms21093141.
The digital polymerase chain reaction (dPCR) is considered to be the third-generation polymerase chain reaction (PCR), as it yields direct, absolute and precise measures of target sequences. dPCR has proven particularly useful for the accurate detection and quantification of low-abundance nucleic acids, highlighting its advantages in cancer diagnosis and in predicting recurrence and monitoring minimal residual disease, mostly coupled with next generation sequencing. In the last few years, a series of studies have employed dPCR for the analysis of hematologic malignancies. In this review, we will summarize these findings, attempting to focus on the potential future perspectives of the application of this promising technology.
数字聚合酶链反应（dPCR）被认为是第三代聚合酶链反应（PCR），因为它可以直接、绝对和精确地测量靶序列。dPCR 已被证明在准确检测和定量低丰度核酸方面特别有用，突出了其在癌症诊断以及预测复发和监测微小残留疾病方面的优势，主要与下一代测序相结合。在过去的几年中，一系列研究已经使用 dPCR 来分析血液系统恶性肿瘤。在这篇综述中，我们将总结这些发现，试图专注于这项有前途的技术的潜在未来应用前景。
