Matsuo T, Matsuo N
Department of Ophthalmology, Okayama University Medical School, Japan.
Jpn J Ophthalmol. 1988;32(4):379-84.
We reported previously familial hypo-retinol-binding proteinemia in a child who developed keratomalacia during measles infection. In the present study, we characterized serum retinol-binding proteins (RBPs) in the affected family members and compared these RBPs with those in the unaffected family members. Immunoblotting following SDS-polyacrylamide gel electrophoresis and isoelectric focusing demonstrated no differences in molecular weight and isoelectric point between the RBPs from the affected and unaffected family members, as far as RBPs were detected by antibody against standard RBP. Gel filtration revealed that all the RBPs in the serum of the affected family members made a complex with prealbumin.
我们之前报道过一名儿童患家族性低视黄醇结合蛋白血症，该患儿在麻疹感染期间发生了角膜软化症。在本研究中，我们对受影响家庭成员的血清视黄醇结合蛋白（RBP）进行了特性分析，并将这些RBP与未受影响家庭成员的RBP进行了比较。SDS-聚丙烯酰胺凝胶电泳和等电聚焦后的免疫印迹显示，就通过抗标准RBP抗体检测到的RBP而言，受影响和未受影响家庭成员的RBP在分子量和等电点上没有差异。凝胶过滤显示，受影响家庭成员血清中的所有RBP均与前白蛋白形成复合物。
