Department of Biochemistry and Molecular Biology, Howard University College of Medicine, Washington, DC, U.S.A.
Anticancer Res. 2020 May;40(5):2559-2565. doi: 10.21873/anticanres.14226.
BACKGROUND/AIM: Androgens are essential for the growth of most prostate cancers (PCa). As a result, androgen ablation is the mainstay of the treatment of PCa. Proteins of the polycomb and trithorax family are master epigenetic regulators of cell type specific gene expression including androgen receptor.
We interrogated epigenetic changes of a 24-gene panel corresponding to polycomb and trithorax genes by PCR array and differential gene expression by quantitative real time-PCR on prostate cancer cell line (LNCaP) treated with the synthetic ligand R1881.
We observed the highest methylation for CBX2, PCGF6, PHC2, EZH2 and TRIM27 genes and the lowest methylation for CBX8 and PCGF2 (p<0.05), and a modest decrease in the expression of EZH2.
Differential methylation profiles of polycomb and trithorax genes may contribute to the dynamics of prostate carcinogenesis.
背景/目的：雄激素是大多数前列腺癌（PCa）生长所必需的。因此，雄激素剥夺是治疗 PCa 的主要方法。多梳和三翼转录因子家族的蛋白是细胞类型特异性基因表达的主要表观遗传调节剂，包括雄激素受体。
我们通过 PCR 阵列检测了与多梳和三翼转录因子基因相对应的 24 个基因的 panel 的表观遗传变化，并通过定量实时 PCR 检测了前列腺癌细胞系（LNCaP）在合成配体 R1881 作用下的差异基因表达。
我们观察到 CBX2、PCGF6、PHC2、EZH2 和 TRIM27 基因的甲基化程度最高，而 CBX8 和 PCGF2 的甲基化程度最低（p<0.05），EZH2 的表达也略有下降。
多梳和三翼转录因子基因的差异甲基化谱可能有助于前列腺癌发生的动态变化。
