Natural Resources Institute Finland (Luke), Natural Resources Unit, Forest Health and Biodiversity, Latokartanonkaari 9, 00790, Helsinki, Finland.
Arch Virol. 2020 Jul;165(7):1679-1682. doi: 10.1007/s00705-020-04642-2. Epub 2020 May 4.
This report describes the complete genome sequence of a double-stranded RNA (dsRNA) virus infecting the oomycetous plant pathogen Phytophthora cactorum. The virus genome consists of a single dsRNA segment of 5699 bp with two open reading frames predicted to overlap with each other and encoding a putative capsid protein of 705 aa and an RNA-dependent RNA polymerase of 779 aa. Sequence comparisons indicated that this virus, designated as "Phytophthora cactorum RNA virus 1" (PcRV1), shares the highest sequence similarity with the unclassified Pythium splendens RNA virus 1 (58% RdRp aa sequence identity). Phylogenetic analysis revealed that these two oomycete viruses group together with Giardia lamblia virus (GVL; family Totiviridae) and several unclassified toti-like viruses from arthropods, fish and fungi. This is the first report of a toti-like virus in a member of the genus Phytophthora and the first virus characterized in P. cactorum.
本报告描述了一种感染卵菌植物病原体可可球二孢菌的双链 RNA(dsRNA)病毒的完整基因组序列。该病毒基因组由一个 5699bp 的单链 dsRNA 片段组成，有两个开放阅读框，预测它们相互重叠，分别编码一个推定的衣壳蛋白（705 个氨基酸）和一个 RNA 依赖性 RNA 聚合酶（779 个氨基酸）。序列比较表明，该病毒被命名为“可可球二孢菌 RNA 病毒 1”（PcRV1），与未分类的灿烂节旋毛孢 RNA 病毒 1（58% RdRp 氨基酸序列同一性）具有最高的序列相似性。系统发育分析表明，这两种卵菌病毒与蓝氏贾第鞭毛虫病毒（GVL；真菌 totiviridae 科）以及来自节肢动物、鱼类和真菌的几种未分类的 tot 样病毒聚集在一起。这是在可可球二孢菌属成员中发现的第一个 tot 样病毒，也是可可球二孢菌中第一个被描述的病毒。
