Infectious Disease Research Centre, CHU de Québec and Laval University, Québec, QC, Canada.
Methods Mol Biol. 2020;2142:9-22. doi: 10.1007/978-1-0716-0581-3_2.
Zika virus (ZIKV) is an important pathogen transmitted to humans by the mosquito vector Aedes aegypti. ZIKV is able to infect several tissues and organs and, importantly, has been associated with microcephaly and central nervous system abnormalities in fetuses and newborn babies of mothers exposed to ZIKV during pregnancy, as well as neurological diseases such as Guillain-Barré syndrome in adults. There is currently no vaccine or drug licensed to prevent or treat ZIKV infections. The use of ZIKV isolation in disease diagnosis has been largely replaced by new techniques. However, virus isolation is still considered as a gold standard for the detection of ZIKV and is usually performed in research and reference laboratories for characterization, sequencing, and a variety of research experiments including pathogenesis, drug susceptibility, and vaccine efficacy. The experimental procedures presented here describe the most common techniques used for ZIKV isolation, propagation, purification, and quantification.
Zika 病毒（ZIKV）是一种由蚊虫传播媒介埃及伊蚊传播给人类的重要病原体。ZIKV 能够感染多种组织和器官，重要的是，已经与怀孕期间接触 ZIKV 的母亲所生胎儿和新生儿的小头畸形和中枢神经系统异常，以及成人的吉兰-巴雷综合征等神经系统疾病有关。目前尚无获准用于预防或治疗 ZIKV 感染的疫苗或药物。疾病诊断中 ZIKV 的分离已在很大程度上被新技术所取代。然而，病毒分离仍然被认为是检测 ZIKV 的金标准，通常在研究和参考实验室中进行，用于特征描述、测序和各种研究实验，包括发病机制、药物敏感性和疫苗功效。本文介绍的实验步骤描述了用于 ZIKV 分离、增殖、纯化和定量的最常见技术。
