RT-qPCR、RT-LAMP 和 RT-RPA 检测 Zika 病毒的比较分析
Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA.
机构信息
Arbovirus and Viral Haemorrhagic Fever Unit, Institut Pasteur de Dakar, Dakar, Senegal.
Institute of Aquaculture, University of Stirling, Stirling, Scotland, UK.
出版信息
Methods Mol Biol. 2020;2142:165-179. doi: 10.1007/978-1-0716-0581-3_14.
Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.
寨卡病毒（ZIKV）的分子检测是疫情管理的关键要素。已经发表了多种针对不同 ZIKV 序列的 PCR 和等温 ZIKV 检测方法。在这项研究中，我们比较了 qRT-PCR、2 种基于不同引物设计方法的 RT-LAMP 检测方法和 RT-RPA，用于检测来自人类、蚊子和猴子的寨卡病毒分离株的非洲和亚洲/美洲谱系。结果表明，RT-LAMP 在时间阈值（Tt）为 18.01±11.71 分钟时检测到 100%的样本，qRT-PCR 在 Tt 为 58.30±16.58 分钟时检测到 88.88%的样本，RT-RPA 在 Tt 为 3.70±0.44 分钟时检测到 50%的样本。
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