Chen J, Li C, Li H, Yu H, Zhang X, Yan M, Guo Y, Yao Z
Department of Dermatology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Institute of Dermatology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Eur Acad Dermatol Venereol. 2021 Jan;35(1):150-158. doi: 10.1111/jdv.16563. Epub 2020 Jul 27.
Psoriasis is an immune-mediated, chronic inflammatory disease with diverse phenotypes. However, its biological diversity has not been well-characterized in Chinese psoriasis population.
To characterize psoriasis biological heterogenicity using gene expression profiles of lesional skin biopsy specimens in a Chinese psoriasis population.
Lesional tissues and blood samples from Chinese psoriasis patients (n = 40), atopic dermatitis (AD) patients (n = 25) and age-matched healthy controls (n = 19) were investigated by using real-time PCR array, histological evaluation and flow cytometry. Unsupervised hierarchical clustering was performed using gene expression profiles of patients with psoriasis.
Two distinct psoriasis clusters were identified. Both clusters indicated high T 17 activation. One cluster (n = 6 of 40 consecutive psoriasis patients) indicated a strong T 2 component in skin lesions, with early onset and low peripheral blood eosinophil level. Significantly higher IL-4, IL-13, IL-25, IL-31 and TSLP gene induction typified this cluster of psoriasis patients, even compared with AD patients. Both psoriasis clusters were characterized by neutrophilic microabscess formation. Histologically, the T 2 high psoriasis cluster indicated a low percentage of perivascular eosinophils.
Two distinct psoriasis clusters were identified. One presented early onset and a low eosinophil level, indicating T 17 polarization and a strong T 2 component. These results laid the foundation for further demonstrating the pathogenesis of psoriasis in Chinese population.
银屑病是一种免疫介导的慢性炎症性疾病，具有多种表型。然而，其生物学多样性在中国银屑病患者群体中尚未得到充分表征。
利用中国银屑病患者群体中皮损活检标本的基因表达谱来表征银屑病的生物学异质性。
采用实时PCR阵列、组织学评估和流式细胞术对中国银屑病患者（n = 40）、特应性皮炎（AD）患者（n = 25）和年龄匹配的健康对照者（n = 19）的皮损组织和血液样本进行研究。使用银屑病患者的基因表达谱进行无监督层次聚类。
识别出两个不同的银屑病聚类。两个聚类均显示T17高度激活。其中一个聚类（40例连续银屑病患者中的6例）在皮损中显示出较强的T2成分，起病早且外周血嗜酸性粒细胞水平低。与AD患者相比，该聚类的银屑病患者中IL-4、IL-13、IL-25、IL-31和TSLP基因诱导水平显著更高。两个银屑病聚类均以嗜中性微脓肿形成为特征。组织学上，T2高的银屑病聚类显示血管周围嗜酸性粒细胞百分比低。
识别出两个不同的银屑病聚类。其中一个起病早且嗜酸性粒细胞水平低，表明T17极化和较强的T2成分。这些结果为进一步阐明中国人群中银屑病的发病机制奠定了基础。
