Zhang Xuan, Liao You-Jun, Liu Zi-Xuan, Wang Rui, Liu Hong-Can, Zheng Yan-Yan, Zhou Yu-Guang, Yu Ya-Jing, Xue Lu, Li Ai-Hua
School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, PR China.
China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006630.
Five aerobic, Gram-stain-negative bacterial strains, designated as C3-2-a3, B3-2-R+30, C3-2-a4, C3-2-M3 and C3-2-M8, were isolated from the coastal soil of LungmuCo Lake in the Tibet Autonomous Region, PR China. Phylogenetic analyses based on 16S rRNA genes and genomes indicated that these isolates belonged to the genus and showed a high similarity to LNNU 24178 (99.01%), RD2P54 (98.80%) and SJ-92 (97.67%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain C3-2-a3 and related reference strains LNNU 24178, RD2P54 and SJ-92 were 91.89, 83.11 and 83.86% and 46.90, 26.90 and 28.20%, respectively. All values were below the thresholds for delineating species, supporting their classification as novel species of the genus . The genomic DNA G+C content of strains C3-2-a3 was 68.39%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The predominant respiratory quinone was ubiquinone-8 (Q-8), aligning with the characteristics of members of the genus . The major fatty acids (>10.0%) of strain C3-2-a3 were identified as iso-C, iso-C, iso-C and iso-C 9. Based on the results of phenotypic, physiological, chemotaxonomic and genotypic characterizations, we propose that the isolates represent a novel species of genus , for which the name p. nov is proposed. The type strain is C3-2-a3 (=CGMCC 1.14507=KCTC 8642).
从中华人民共和国西藏自治区龙木错湖的海岸土壤中分离出五株需氧、革兰氏染色阴性的细菌菌株，分别命名为C3-2-a3、B3-2-R+30、C3-2-a4、C3-2-M3和C3-2-M8。基于16S rRNA基因和基因组的系统发育分析表明，这些分离株属于该属，并且与LNNU 24178（99.01%）、RD2P54（98.80%）和SJ-92（97.67%）具有高度相似性。菌株C3-2-a3与相关参考菌株LNNU 24178、RD2P54和SJ-92之间的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为91.89%、83.11%和83.86%以及46.90%、26.90%和28.2%。所有值均低于划分物种的阈值，支持将它们分类为该属的新物种。菌株C3-2-a3的基因组DNA G+C含量为68.39%。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的磷脂。主要呼吸醌为泛醌-8（Q-8），与该属成员的特征一致。菌株C3-2-a3的主要脂肪酸（>10.0%）被鉴定为异-C、异-C、异-C和异-C 9。基于表型、生理、化学分类和基因型特征的结果，我们提议这些分离株代表该属的一个新物种，为此提出新名称p. nov。模式菌株为C3-2-a3（=CGMCC 1.14507=KCTC 8642）。
