Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 355 Science Road, Daejeon 34141, Republic of Korea.
Biomedical Convergence Science & Technology, Industrial Technology Advances, Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea.
Nano Lett. 2020 Jun 10;20(6):4708-4713. doi: 10.1021/acs.nanolett.0c00715. Epub 2020 May 11.
As a promising tool over the optical resolution limits, liquid electron microscopy is practically utilized to visualize the structural information on wet biological specimens, such as cells, proteins, and nucleic acids. However, the functionality of biomolecules during their observation is still controversial. Here we show the feasibility of live-cell electron microscopy using graphene veils. We demonstrate that the electron dose resistivity of live bacterial cells increases to 100-fold in graphene veils, and thus they maintain their structures and functions after electron microscopy experiments. Our results provide the guidelines and show possibilities for the electron microscopy imaging of live cells and functional biomolecules.
作为一种超越光学分辨率限制的有前途的工具，液相电子显微镜被实际用于可视化湿生物样本（如细胞、蛋白质和核酸）的结构信息。然而，在观察过程中生物分子的功能仍然存在争议。在这里，我们展示了使用石墨烯面纱进行活细胞电子显微镜观察的可行性。我们证明了活细菌细胞的电子剂量电阻在石墨烯面纱中增加了 100 倍，因此它们在电子显微镜实验后仍然保持其结构和功能。我们的结果为活细胞和功能生物分子的电子显微镜成像提供了指导方针和可能性。
