Park Chan Soon, Kim Inho, Oh Gyu Chul, Han Jung-Kyu, Yang Han-Mo, Park Kyung Woo, Cho Hyun-Jai, Kang Hyun-Jae, Koo Bon-Kwon, Chung Woo-Young, Oh Seil, Lee Hae-Young
Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea.
Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul 03080, Korea.
J Clin Med. 2020 May 2;9(5):1313. doi: 10.3390/jcm9051313.
We investigated the diagnostic value and pathophysiological role of circulating microRNA (miR) in vasospastic angina (VA). We enrolled patients who underwent coronary angiography for chest pain to explore the miR's diagnostic utility. In addition, we investigated the role of miRs in regulating endothelial nitric oxide synthase (eNOS) expression in human coronary artery endothelial cells (hCAECs). Among the 121 patients, 46 were diagnosed with VA (VA group), 26 with insignificant coronary lesions (ICL group), and 49 with atherothrombotic angina (AA group). The VA group showed a significantly higher expression of miR-17-5p, miR-92a-3p, and miR-126-3p than the ICL group. In contrast, miR-221-3p and miR-222-3p were upregulated in the AA group compared to the VA group, and all levels of miR-17-5p, miR-92a-3p, miR-126-3p, miR-145-5p, miR-221-3p, and miR-222-3p differed between the AA group and the ICL group. In the hCAECs, transfection with mimics (pre-miR) of miR-17-5p, miR-92a-3p, and miR-126-3p was associated with eNOS suppression. Additionally, transfection with inhibitors (anti-miR) of miR-92a-3p significantly rescued the eNOS suppression induced by lipopolysaccharide. In conclusion, the circulating miRs not only proved to have diagnostic utility, but also contributed to pathogenesis by eNOS regulation.
我们研究了循环微RNA（miR）在血管痉挛性心绞痛（VA）中的诊断价值及病理生理作用。我们纳入了因胸痛接受冠状动脉造影的患者，以探讨miR的诊断效用。此外，我们研究了miR在调节人冠状动脉内皮细胞（hCAECs）中内皮型一氧化氮合酶（eNOS）表达方面的作用。在121例患者中，46例被诊断为VA（VA组），26例为冠状动脉病变不显著（ICL组），49例为动脉粥样硬化血栓形成性心绞痛（AA组）。VA组中miR-17-5p、miR-92a-3p和miR-126-3p的表达显著高于ICL组。相比之下，与VA组相比，AA组中miR-221-3p和miR-222-3p上调，并且AA组与ICL组之间miR-17-5p、miR-92a-3p、miR-126-3p、miR-145-5p、miR-221-3p和miR-222-3p的所有水平均存在差异。在hCAECs中，用miR-17-5p、miR-92a-3p和miR-126-3p的模拟物（pre-miR）转染与eNOS抑制相关。此外，用miR-92a-3p的抑制剂（抗miR）转染可显著挽救脂多糖诱导的eNOS抑制。总之，循环miR不仅被证明具有诊断效用，还通过调节eNOS参与了发病机制。
