Department of Biomedical Sciences and Biotechnology (BIOMETEC), University of Catania, Catania, Italy.
Department of Clinical and Experimental Medicine (MEDCLIN), University of Catania, Catania, Italy.
Nat Prod Res. 2021 Dec;35(23):5354-5359. doi: 10.1080/14786419.2020.1761355. Epub 2020 May 6.
Citrus fruits are rich sources of bioactive compounds and their consumption is associated to health-promoting effects. Citrus processing wastes contain bioflavonoids and other high added value compounds. The aim of this study was to evaluate the antiallergic properties of a new phytoextract obtained by citrus wastes and peels. Blood orange and lemon processing wastes were used to produce a Red orange and Lemon Extract (RLE). Blood samples from 30 allergic donors were collected and used to evaluate the basophil activation (CD203c) and degranulation (CD63) by stimulation trough allergen with and without the RLE. Reduced basophil expression of CD203c and CD63 were observed in RLE + Allergen treated samples, with -20.21% of CD203c expression ( < 0.0001) and -54.11% of CD63 expression ( < 0.0001), compared to Allergen treated samples. The RLE evidenced a good antiallergic activity, mainly acting on basophils degranulation, and therefore reducing the key event of pro-inflammatory mediators release after allergic stimuli.
柑橘类水果是生物活性化合物的丰富来源，其消费与促进健康的作用有关。柑橘加工废物含有类黄酮和其他高附加值化合物。本研究旨在评估一种新的植物提取物的抗过敏特性，该提取物由柑橘废物和果皮制成。使用血橙和柠檬加工废物生产了一种红橙和柠檬提取物（RLE）。从 30 名过敏供体采集血液样本，并通过刺激过敏原和 RLE 来评估嗜碱性粒细胞的活化（CD203c）和脱颗粒（CD63）。在 RLE+过敏原处理的样品中观察到嗜碱性粒细胞 CD203c 和 CD63 的表达减少，与过敏原处理的样品相比，CD203c 的表达减少了-20.21%（<0.0001），CD63 的表达减少了-54.11%（<0.0001）。RLE 表现出良好的抗过敏活性，主要作用于嗜碱性粒细胞脱颗粒，从而减少过敏刺激后促炎介质释放的关键事件。
