Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile; Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago de Chile 8380453, Chile.
Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile.
Biomed Pharmacother. 2020 Jul;127:110178. doi: 10.1016/j.biopha.2020.110178. Epub 2020 May 3.
Chagas disease, caused by the protozoan parasiteTrypanosoma cruzi, has no effective treatment available. On the other hand, microalgae are aquatic organisms that constitute an interesting reservoir of biologically active metabolites. Moreover, some species of green and red algae present anti-protozoan activity. Our aim was to study the antiparasitic effects of aqueous, methanolic and ethanolic extracts from different microalgae.
Our results show that the methanolic extracts of S. obliquus and T. suecica as well as the ethanolic extracts of C. reinhardtii and T. suecica present trypanocidal activity on the infective extracellular trypomastigotes and intracellular amastigotes. In addition, the ethanolic extract of C. reinhardtii potentiates the activity of the conventional antichagasic drug nifurtimox. In order to identify some potential compounds with trypanocidal activity, we performed a phytochemical screening analyzing the presence of phenolic compounds, pigments and terpenoids.
The different microalgae extracts, particularly the ethanolic extract ofC. reinhardtii, are promising potential candidates for the development of future natural antichagasic drugs.
由原生动物寄生虫克氏锥虫引起的恰加斯病目前尚无有效治疗方法。另一方面，微藻是水生生物，构成了具有生物活性代谢物的有趣库。此外，一些绿藻和红藻具有抗原生动物活性。我们的目的是研究来自不同微藻的水提物、甲醇提物和乙醇提物的抗寄生虫作用。
我们的结果表明，斜生栅藻和瑞典衣藻的甲醇提取物以及莱茵衣藻和瑞典衣藻的乙醇提取物对感染性细胞外锥虫和细胞内无鞭毛体均具有杀锥虫活性。此外，莱茵衣藻的乙醇提取物增强了常规抗恰加斯病药物硝呋替莫的活性。为了鉴定一些具有杀锥虫活性的潜在化合物，我们进行了植物化学筛选，分析了酚类化合物、色素和萜类化合物的存在。
不同的微藻提取物，特别是莱茵衣藻的乙醇提取物，是开发未来天然抗恰加斯病药物的有前途的潜在候选物。
