Division of Toxicology, Office of Applied Research and Safety Assessment, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration (FDA), 8301 Muirkirk Rd, Laurel, MD, 20708, USA.
Anal Bioanal Chem. 2020 Jul;412(17):3975-3982. doi: 10.1007/s00216-020-02681-x. Epub 2020 May 6.
In vivo, drug molecules interact with their biological targets (e.g., enzymes, receptors, ion channels, transporters), thereby eliciting therapeutic effects. Assays that measure the interaction between drugs and bio-targets may be used as drug biosensors, which are capable of broadly detecting entire drug classes without prior knowledge of their chemical structure. This Trends article covers recent developments in bio-target-based screening assays for detecting drugs associated with the following areas: illicit products marketed as dietary supplements, food-producing animals, and bodily fluids. General challenges and considerations associated with using bio-target assays are also presented. Finally, future applications of these assays for drug detection are suggested based upon current needs.
在体内，药物分子与它们的生物靶标（例如酶、受体、离子通道、转运体）相互作用，从而产生治疗效果。测量药物与生物靶标之间相互作用的测定法可用作药物生物传感器，其能够广泛检测整个药物类别，而无需事先了解其化学结构。本文综述了基于生物靶标的筛选测定法在检测与以下领域相关药物方面的最新进展：被作为膳食补充剂销售的非法产品、食用动物和体液。还提出了使用生物靶标测定法所涉及的一般挑战和注意事项。最后，根据当前的需求，提出了这些测定法在药物检测方面的未来应用。
