Arnanz Ana, De Munck Neelke, Bayram Aşina, El-Damen Ahmed, Abdalla Andrea, ElKhatib Ibrahim, Melado Laura, Lawrenz Barbara, Fatemi Human M
IVIRMA Middle East Fertility Clinic, Abu Dhabi, United Arab Emirates.
J Assist Reprod Genet. 2020 Jun;37(6):1387-1397. doi: 10.1007/s10815-020-01795-6. Epub 2020 May 6.
To evaluate whether mtDNA content at the blastocyst stage differs between embryos derived from fresh or vitrified sibling oocytes.
A retrospective analysis was performed between March 2017 and September 2018, including 504 blastocysts from 94 couples undergoing preimplantation genetic testing for aneuploidies (PGT-A), using fresh oocytes together with previously vitrified oocytes. Trophectoderm biopsies were performed and subjected to next generation sequencing.
On average, 1.8 ± 1.0 oocyte vitrification cycles were performed per patient. Between fresh and vitrified cycles, no difference was observed between the number of fertilized oocytes (5.3 ± 4.2 versus 5.5 ± 3.0). Blastulation rate on day 5 per fertilized oocyte was significantly higher in the fresh group (62% ± 29% versus 44% ± 31%; p < 0.001). For the 504 biopsied blastocysts, 294 fresh versus 210 vitrified, no significant differences were found in the euploid rate, 40.5% versus 38.6% (p = 0.667), and mtDNA content, 30.1 (± 10.6) versus 30.0 (± 12.5) (p = 0.871), respectively. Regardless of the origin of the oocytes, aneuploid blastocysts contained significantly higher mtDNA values compared with the euploid ones (31.4 versus 28.0; p = 0.001). Furthermore, top-quality blastocysts had a significantly lower mtDNA content compared with moderate and poor-quality blastocysts (p < 0.001) and blastocysts biopsied on day 5 showed significantly lower mtDNA content compared with day 6 or day 7 blastocysts (p < 0.001). However, when analyzing the blastocyst mtDNA content according to the ploidy state, no differences were found for blastocyst quality or day of biopsy between blastocysts originating from fresh or vitrified oocytes.
Oocyte vitrification does not affect the mtDNA content of trophectoderm biopsies.
评估新鲜或玻璃化处理的同胞卵母细胞来源的胚胎在囊胚阶段的线粒体DNA（mtDNA）含量是否存在差异。
对2017年3月至2018年9月期间进行回顾性分析，纳入94对接受非整倍体植入前基因检测（PGT-A）的夫妇的504个囊胚，使用新鲜卵母细胞以及先前玻璃化处理的卵母细胞。进行滋养外胚层活检并进行下一代测序。
每位患者平均进行1.8±1.0次卵母细胞玻璃化处理周期。在新鲜周期和玻璃化周期之间，受精的卵母细胞数量没有差异（5.3±4.2对5.5±3.0）。新鲜组中每个受精卵母细胞在第5天的囊胚形成率显著更高（62%±29%对44%±31%；p<0.001）。对于504个活检囊胚，294个来自新鲜卵母细胞，210个来自玻璃化处理的卵母细胞，整倍体率分别为40.5%对38.6%（p=0.667），mtDNA含量分别为30.1（±10.6）对30.0（±12.5）（p=0.871），均未发现显著差异。无论卵母细胞来源如何，非整倍体囊胚的mtDNA值均显著高于整倍体囊胚（31.4对28.0；p=0.001）。此外，优质囊胚的mtDNA含量显著低于中等质量和低质量囊胚（p<0.001），第5天活检的囊胚的mtDNA含量显著低于第6天或第7天活检的囊胚（p<0.001）。然而，根据倍性状态分析囊胚mtDNA含量时，来自新鲜或玻璃化处理卵母细胞的囊胚在囊胚质量或活检日期方面未发现差异。
卵母细胞玻璃化处理不影响滋养外胚层活检的mtDNA含量。
