ART Fertility Clinics, Abu Dhabi, P.O. Box 60202, United Arab Emirates.
Obstetrical Department, Women's University Hospital Tübingen, Tübingen, Germany.
J Assist Reprod Genet. 2021 Mar;38(3):595-604. doi: 10.1007/s10815-020-02050-8. Epub 2021 Jan 4.
To determine whether the blastocyst mitochondrial DNA (mtDNA) content is related to the miscarriage rate in patients undergoing single euploid frozen embryo transfer (SEFET).
A total of 355 single euploid frozen embryo transfer cycles were studied retrospectively between April 2017 and December 2018. A trophectoderm biopsy was performed on day 5/6 blastocysts. Post next-generation sequencing (NGS), the mtDNA content was calculated as the ratio of mitochondrial DNA over nuclear DNA, and the association between blastocyst mtDNA content and miscarriage rate was evaluated.
RESULT(S): Three hundred fifty-five euploid blastocysts were selected for SEFET in 314 patients with an average age of 33.7 ± 5.6 years; 255 were biopsied on day 5 (71.8%) and 100 on day 6 (28.2%). Frozen embryo transfer (FET) was performed either in a hormone replacement therapy (HRT) cycle (71.8%; n = 255) or in a natural cycle (NC) (28.2%; n = 100). A pregnancy rate of 66.2% (235/355) was obtained with clinical pregnancy and miscarriage rates of 52.4% (n = 186) and 5.6% (n = 20), respectively. There was no significant difference neither between the blastocyst mtDNA content of pregnant and nonpregnant patients (27.7 ± 9.2 vs. 29.4 ± 8.6, P = 0.095) nor between patients with a clinical pregnancy and miscarriage (30.5 ± 9.3 vs. 27.3 ± 9.2, P = 0.136). Multivariate logistic regression analysis showed the same nonsignificant relationship, except for the miscarriage rate and BMI (OR 1.149, 95% CI 1.03-1.28; P = 0.012).
CONCLUSION(S): Mitochondrial DNA content is unable to predict the miscarriage of implanted human euploid blastocysts.
确定进行单整倍体冷冻胚胎移植（SEFET）的患者中囊胚线粒体 DNA（mtDNA）含量与流产率之间的关系。
回顾性分析 2017 年 4 月至 2018 年 12 月期间进行的 355 例单整倍体冷冻胚胎移植周期。第 5/6 天对滋养外胚层活检进行检测。通过下一代测序（NGS）后，mtDNA 含量通过线粒体 DNA 与核 DNA 的比值计算，评估囊胚 mtDNA 含量与流产率之间的关系。
314 名患者的 355 个整倍体囊胚被选择进行 SEFET，平均年龄为 33.7±5.6 岁；255 个在第 5 天（71.8%）活检，100 个在第 6 天（28.2%）活检。冷冻胚胎移植（FET）在激素替代治疗（HRT）周期中进行（71.8%；n=255）或自然周期（NC）中进行（28.2%；n=100）。获得了 66.2%（235/355）的妊娠率，临床妊娠和流产率分别为 52.4%（n=186）和 5.6%（n=20）。妊娠患者和未妊娠患者的囊胚 mtDNA 含量无显著差异（27.7±9.2 与 29.4±8.6，P=0.095），临床妊娠患者和流产患者的囊胚 mtDNA 含量也无显著差异（30.5±9.3 与 27.3±9.2，P=0.136）。多变量逻辑回归分析显示，除了流产率与 BMI（OR 1.149，95%CI 1.03-1.28；P=0.012）之间存在相同的无显著关系外，结果相同。
线粒体 DNA 含量不能预测植入的人类整倍体囊胚的流产。
