Department of Ophthalmology, University of Würzburg, Josef-Schneider-Straße 11, 97080, Würzburg, Germany.
Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Graefes Arch Clin Exp Ophthalmol. 2020 Jun;258(6):1211-1216. doi: 10.1007/s00417-020-04691-y. Epub 2020 May 5.
To characterize the effects of netarsudil on the aqueous humor outflow tract distal to the trabecular meshwork (TM). We hypothesized that netarsudil increases outflow facility in eyes with and without circumferential ab interno trabeculectomy (AIT) that removes the TM.
Sixty-four porcine anterior segment cultures were randomly assigned to groups with (n = 32) and without circumferential AIT (n = 32). Cultures were exposed to 0.1, 1, and 10 μM netarsudil (N = 8 eyes per concentration). For each concentration, IOP and vessel diameters were compared with their respective pretreatment baselines. Outflow tract vessel diameters were assessed by spectral-domain optical coherence tomography (SDOCT) and rendered in 4D (XYZ time series).
Netarsudil at 1 μM reduced IOP both in eyes with TM (- 0.60 ± 0.24 mmHg, p = 0.01) and in eyes without TM (- 1.79 ± 0.42 mmHg, p < 0.01). At this concentration, vessels of the distal outflow tract dilated by 72%. However, at 0.1 μM netarsudil elevated IOP in eyes with TM (1.59 ± 0.36 mmHg, p < 0.001) as well as in eyes without TM (0.23 ± 0.32 mmHg, p < 0.001). Vessels of the distal outflow tract constricted by 31%. Similarly, netarsudil at a concentration of 10 μM elevated IOP both in eyes with TM (1.91 ± 0.193, p < 0.001) and in eyes without TM (3.65 ± 0.86 mmHg, p < 0.001). At this concentration, outflow tract vessels constricted by 27%.
In the porcine anterior segment culture, the dose-dependent IOP changes caused by netarsudil matched the diameter changes of distal outflow tract vessels. Hyper- and hypotensive properties of netarsudil persisted after TM removal.
描述奈塔舒利对小梁网后房水流出道的作用。我们假设奈塔舒利能增加有和没有环内小梁切除术（AIT）的眼睛的房水流出率，因为 AIT 去除了小梁网。
64 个猪眼前节培养物被随机分为有（n=32）和没有（n=32）环内 AIT 的两组。培养物暴露于 0.1、1 和 10 μM 的奈塔舒利（每种浓度 8 只眼）。对于每种浓度，IOP 和血管直径与各自的预处理基线进行比较。房水流出道血管直径通过光谱域光学相干断层扫描（SDOCT）评估，并在 4D（XYZ 时间序列）中呈现。
1 μM 的奈塔舒利降低了有小梁网的眼睛（-0.60±0.24mmHg，p=0.01）和没有小梁网的眼睛（-1.79±0.42mmHg，p<0.01）的 IOP。在这个浓度下，远端流出道的血管扩张了 72%。然而，在 0.1 μM 的奈塔舒利时，有小梁网的眼睛的 IOP 升高（1.59±0.36mmHg，p<0.001）和没有小梁网的眼睛的 IOP 升高（0.23±0.32mmHg，p<0.001）。远端流出道的血管收缩了 31%。同样，10 μM 的奈塔舒利在有小梁网的眼睛（1.91±0.193mmHg，p<0.001）和没有小梁网的眼睛（3.65±0.86mmHg，p<0.001）中升高了 IOP。在这个浓度下，流出道的血管收缩了 27%。
在猪眼前节培养物中，奈塔舒利引起的 IOP 变化与远端流出道血管的直径变化呈剂量依赖性。奈塔舒利的高和低血压特性在去除小梁网后仍然存在。
