Dental School, Dental Research Center, Research Institute for Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Cell Physiol. 2020 Dec;235(12):9568-9576. doi: 10.1002/jcp.29764. Epub 2020 May 5.
Periodontitis is a complex disorder that affects a large number of human beings from different ethnic groups. This condition has been associated with dysregulation of a number of genes, among them are long noncoding RNAs (lncRNAs). In the current study, we assessed the expression of four lncRNAs (BDNF-AS, MIAT, MIR137HG, and PNKY) as well as BDNF in the peripheral blood and gingival tissues obtained from patients with periodontitis and healthy subjects. The expression of BDNF was significantly lower in blood samples of male patients with periodontitis compared with male controls (posterior β of RE = -4.754, p = .048). However, there was no significant difference in the expression of BDNF in tissue samples from the cases and controls. The expression of BDNF-AS was significantly lower in the tissue samples of patients compared with control tissue samples (posterior β of RE = -2.151, p = .019). Such an expression difference was detected between male subgroups as well (posterior β of RE = -3.679, p = .009). However, expression of this lncRNA was not different in blood samples obtained from patients compared with healthy subjects. The expression of PNKY was significantly higher in tissue samples obtained from female patients compared with sex-matched controls (posterior β of RE = 6.23, p = .037). Blood levels of this lncRNA were not different between cases and controls. There was no significant difference either in the tissue expression or in blood expression of MIR137HG or MIAT between cases and controls. The current study indicates the putative role of BDNF, BDNF-AS, and PNKY in the pathophysiology of periodontitis and potentiates these genes as candidates for functional studies.
牙周炎是一种影响来自不同种族的大量人群的复杂疾病。这种情况与许多基因的失调有关，其中包括长非编码 RNA（lncRNA）。在目前的研究中，我们评估了来自牙周炎患者和健康受试者的外周血和牙龈组织中四种 lncRNA（BDNF-AS、MIAT、MIR137HG 和 PNKY）以及 BDNF 的表达。与男性对照组相比，男性牙周炎患者的血液样本中 BDNF 的表达显著降低（RE 的后β=−4.754，p=0.048）。然而，病例和对照组组织样本中 BDNF 的表达没有显著差异。与对照组织样本相比，患者的组织样本中 BDNF-AS 的表达显著降低（RE 的后β=−2.151，p=0.019）。这种表达差异也在男性亚组中检测到（RE 的后β=−3.679，p=0.009）。然而，与健康受试者相比，从患者中获得的血液样本中该 lncRNA 的表达没有差异。与性别匹配的对照组相比，女性患者的组织样本中 PNKY 的表达显著升高（RE 的后β=6.23，p=0.037）。该 lncRNA 的血液水平在病例和对照组之间没有差异。病例和对照组之间，在组织表达或血液表达 MIR137HG 或 MIAT 方面没有显著差异。本研究表明 BDNF、BDNF-AS 和 PNKY 在牙周炎的病理生理学中具有潜在作用，并增强了这些基因作为功能研究候选者的地位。
