Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena, Humboldtstraße 10, 07743 Jena, Germany.
Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena, Philosophenweg 7, 07743 Jena, Germany.
Anal Chem. 2020 Jun 2;92(11):7932-7939. doi: 10.1021/acs.analchem.0c01323. Epub 2020 May 19.
The large volume and diversified nanomedicine market, undergoing a rapid growth, relies not only on the creation and applicative exploration of nanocarrier-based medicines showing significant potential, but in particular, demands a quantitative assessment of their physicochemical properties. In this study, we demonstrate the in situ assessment of multifunctional biodegradable nanoparticle (NP) entries as core components of nanoscale drug delivery systems (NDDSs) by making use of analytical ultracentrifugation (AUC). We determine and elucidate the following characteristics of NPs in NDDSs: NP density and size, targeting dye functionality, encapsulated and free drug, surfactant, and also NP drug release dynamics, quantitatively interconnected to NP degradation. In concept, we demonstrate this by multidetection AUC experiments at variable speed and time profiles. We could verify the quantitative and accurate nature of AUC for assessment of NDDSs, that is, also future nanomedicines. This concerns modeled and real life solution application formats such as cell culture media and human serum.
庞大且多样化的纳米医学市场正在快速增长，不仅依赖于具有显著潜力的基于纳米载体药物的创新和应用探索，还特别需要对其物理化学性质进行定量评估。在这项研究中，我们通过分析超速离心（AUC）原位评估多功能可生物降解纳米颗粒（NP）作为纳米药物递送系统（NDDS）的核心组件。我们确定并阐明了 NDDS 中 NPs 的以下特性：NP 密度和尺寸、靶向染料功能、包封和游离药物、表面活性剂，以及 NP 药物释放动力学，与 NP 降解定量相关联。从概念上讲，我们通过在不同速度和时间分布下进行多检测 AUC 实验来证明这一点。我们可以验证 AUC 用于评估 NDDS 的定量和准确性，也可以用于未来的纳米药物。这涉及到模型和实际生活中的溶液应用格式，如细胞培养基和人血清。
