Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905 São Carlos, São Paulo, Brazil.
Unidade de Biotecnologia, Universidade de Ribeirão Preto, Avenida Costábile Romano 2201, 14096-900 Ribeirão Preto, São Paulo, Brazil.
J Nat Prod. 2020 May 22;83(5):1409-1415. doi: 10.1021/acs.jnatprod.9b00607. Epub 2020 May 6.
and were obtained by germination from seeds, and isotopic-labeling experiments using d-[1-C]glucose were performed with the seedlings. After 60 days, the seedlings were analyzed by high-performance liquid chromatography-ultraviolet-solid-phase extraction-nuclear magnetic resonance, data and the C enrichment patterns of xanthyletin and seselin indicated that the pyran ring was formed by the methylerythritol phosphate pathway and that the coumarin moiety was derived from the shikimate pathway in both compounds. This information regarding the biosynthetic pathway can be used to increase resistance against phytopathogens, because xanthyletin and seselin are reported to have antimicrobial activity on the growth of , which causes citrus variegated chlorosis in orange.
和 通过种子萌发获得，并用 d-[1-C]葡萄糖进行了同位素标记实验。60 天后，通过高效液相色谱-紫外固相萃取-核磁共振分析了幼苗，黄桐素和香豆素的 C 富集模式表明，吡喃环是通过甲基赤藓醇磷酸途径形成的，而香豆素部分是两种化合物中都来自莽草酸途径。关于生物合成途径的这一信息可用于提高对植物病原体的抗性，因为黄桐素和香豆素有报道称对导致橙子柑橘斑驳黄化的 的生长具有抗菌活性。
