Jang Young-Jin, Lee Dongbin, Hossain Mohammad Amjad, Aravinthan Adithan, Kang Chang-Won, Kim Nam Soo, Kim Jong-Hoon
College of Veterinary Medicine, Biosafety Research Institute, Jeonbuk National University, Iksan-city, Republic of Korea.
College of Veterinary Medicine, Gyeongsang National University, Jinju, Republic of Korea.
J Ginseng Res. 2020 May;44(3):483-489. doi: 10.1016/j.jgr.2019.03.002. Epub 2019 Mar 14.
Korean Red Ginseng (KRG) has been known to possess many ginsenosides. These ginsenosides are used for curing cardiovascular problems. The present study show the protective potential of KRG against doxorubicin (DOX)-induced myocardial dysfunction, by assessing electrocardiographic, hemodynamic, and biochemical parameters and histopathological findings.
Animals were fed a standard chow and adjusted to their environment for 3 days before the experiments. Next, the rats were equally divided into five groups (n = 9, each group). The animals were administered with KRG (250 and 500 mg/kg) for 10 days and injected with DOX (20 mg/kg, subcutaneously, twice at a 24-h interval) on the 8th and 9th day. Electrocardiography and echocardiography were performed to study hemodynamics. Plasma levels of superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde were measured. In addition, the dose of troponin I and activity of myeloperoxidase in serum and cardiac tissue were analyzed, and the histopathological findings were evaluated using light microscopy.
Administration of KRG at a dose of 250 and 500 mg/kg recovered electrocardiographic changes, ejection fraction, fractional shortening, left ventricular systolic pressure, the maximal rate of change in left ventricle contraction (+dP/dt), and left ventricle relaxation (-dP/dt). In addition, KRG treatment significantly normalized the oxidative stress markers in plasma, dose dependently. In addition, the values of troponin I and myeloperoxidase were ameliorated by KRG treatment, dose dependently. And, KRG treatment showed better histopathological findings when compared with the DOX control group.
These mean that KRG mitigates myocardial damage by modulating the hemodynamics, histopathological abnormality, and oxidative stress related to DOX-induced cardiomyopathy in rats. The results of the present study show protective effects of KRG on cardiac toxicity.
已知韩国红参（KRG）含有多种人参皂苷。这些人参皂苷用于治疗心血管问题。本研究通过评估心电图、血流动力学、生化参数和组织病理学结果，显示了韩国红参对阿霉素（DOX）诱导的心肌功能障碍的保护潜力。
在实验前，动物喂食标准饲料并适应环境3天。接下来，将大鼠平均分为五组（每组n = 9）。动物连续10天给予韩国红参（250和500mg/kg），并在第8天和第9天皮下注射DOX（20mg/kg，间隔24小时注射两次）。进行心电图和超声心动图检查以研究血流动力学。测量血浆中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和丙二醛的水平。此外，分析血清和心脏组织中肌钙蛋白I的剂量和髓过氧化物酶的活性，并使用光学显微镜评估组织病理学结果。
给予250和500mg/kg剂量的韩国红参可恢复心电图变化、射血分数、缩短分数、左心室收缩压、左心室收缩最大变化率（+dP/dt）和左心室舒张（-dP/dt）。此外，韩国红参治疗可使血浆中的氧化应激标志物显著恢复正常，呈剂量依赖性。此外，韩国红参治疗可使肌钙蛋白I和髓过氧化物酶的值剂量依赖性改善。并且，与DOX对照组相比，韩国红参治疗显示出更好的组织病理学结果。
这些表明韩国红参通过调节与DOX诱导的大鼠心肌病相关的血流动力学、组织病理学异常和氧化应激来减轻心肌损伤。本研究结果显示了韩国红参对心脏毒性的保护作用。
