Kopalli Spandana Rajendra, Hwang Seock-Yeon, Won Yu-Jin, Kim Sung-Won, Cha Kyu-Min, Han Chang-Kyun, Hong Jae-Yup, Kim Si-Kwan
Department of Life Science, College of Biomedical & Health Science, Konkuk University, Chungju 380-701, Republic of Korea.
Department of Biomedical Laboratory Science, College of Applied Science and Industry, Daejeon University, Daejeon 300-716, Republic of Korea.
Exp Gerontol. 2015 Sep;69:94-102. doi: 10.1016/j.exger.2015.05.004. Epub 2015 May 14.
Distortion of intracellular oxidant and antioxidant balances appears to be a common feature that underlies in age-related male sexual impairment. Therefore regulating oxidative defense mechanisms might be an ideal approach in improving male sexual dysfunctions. In the present study, the effect of Korean red ginseng aqueous extract (KRG) on age-induced testicular dysfunction in rats was investigated. KRG (200mg/kg) mixed with regular pellet diet was administered orally for six months and the morphological, spermatogenic and antioxidant enzyme status in testis of aged rats (18months) were evaluated. Data indicated a significant change in morphology and decrease in spermatogenesis-related parameters in aged rats (AC) compared with young rats (YC). Sperm number, germ cell count, Sertoli cell count and Sertoli cell index were significantly (p<0.05) restored in KRG-treated aged rat groups (G-AC). Further the increased lipid peroxidation as measured by malondialdehyde (p<0.05), and altered enzymatic (superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and catalase) and non-enzymatic (reduced glutathione, ascorbic acid and α-tocopherol) antioxidants (p<0.05) were attenuated by KRG treatment in aged rats to near normal levels as in YC groups. Furthermore, proteomic analysis demonstrated differential expression of selected proteins such as phosphatidylinositol transfer protein, fatty acid binding protein-9, triosephosphate isomerase-1 and aldehyde (aldose) reductase-1in aged rats was significantly (p<0.05) protected by KRG treatment. In conclusion, long-term administration of KRG restored aging-induced testicular ineffectiveness in rats by modulating redox proteins and oxidative defense mechanisms.
细胞内氧化剂和抗氧化剂平衡的失调似乎是与年龄相关的男性性功能障碍的一个共同特征。因此，调节氧化防御机制可能是改善男性性功能障碍的理想方法。在本研究中，研究了韩国红参水提取物（KRG）对大鼠年龄诱导的睾丸功能障碍的影响。将KRG（200mg/kg）与常规颗粒饲料混合后口服给药六个月，并评估老年大鼠（18个月）睾丸的形态、生精和抗氧化酶状态。数据表明，与年轻大鼠（YC）相比，老年大鼠（AC）的形态有显著变化，生精相关参数下降。在KRG处理的老年大鼠组（G-AC）中，精子数量、生殖细胞计数、支持细胞计数和支持细胞指数显著（p<0.05）恢复。此外，通过丙二醛测量的脂质过氧化增加（p<0.05），以及酶促（超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶、谷胱甘肽还原酶和过氧化氢酶）和非酶促（还原型谷胱甘肽、抗坏血酸和α-生育酚）抗氧化剂的改变（p<0.05），在老年大鼠中通过KRG处理减弱至接近YC组的正常水平。此外，蛋白质组学分析表明，老年大鼠中所选蛋白质如磷脂酰肌醇转移蛋白、脂肪酸结合蛋白-9、磷酸丙糖异构酶-1和醛（醛糖）还原酶-1的差异表达通过KRG处理得到显著（p<0.05）保护。总之，长期给予KRG通过调节氧化还原蛋白和氧化防御机制恢复了衰老诱导的大鼠睾丸功能障碍。
