Oguntibeju Oluwafemi O, Aboua Yapo, Kachepe Prisca
Phytomedicine and Phytochemistry Research Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health & Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, 7535, South Africa.
Department of Health Sciences, Faculty of Health and Applied Sciences, Namibia University of Science and Technology, Private Bag, 13388, Windhoek, Namibia.
Heliyon. 2020 Apr 28;6(4):e03817. doi: 10.1016/j.heliyon.2020.e03817. eCollection 2020 Apr.
The study evaluated the effects of vindoline on testicular and epididymal oxidative stress in diabetes-induced male Wistar rats.
Forty-eight (48), 6-week old male Wistar rats weighing between 190-230g were divided into 6 groups (n = 8) and used for this study. Group 1 was the normal control, group 2 comprised non-diabetic rats treated with vindoline, and group 3 was the non-diabetic group of rats treated with glibenclamide-the standard drug for the treatment of diabetes. Group 4 was the diabetic control, group 5 comprised diabetic rats treated with vindoline and group 6 was the diabetic rats treated with glibenclamide. Diabetes was induced in group 4, group 5 and group 6 rats by subjecting them to 10% fructose water over a period of 2 weeks, followed by administration of a single intraperitoneal injection of 40 mg/kg b.w streptozotocin (STZ). Testicular and epididymal lipid peroxidation levels, antioxidant enzymes, scavenging activity and total antioxidant capacity were measured.
Diabetes-induced male Wistar rats demonstrated chronic hyperglycaemia, oxidative stress and reduced oxygen radical absorption capacity in both testicular and epididymal tissues. Short-term treatment of diabetic rats with vindoline for 5 weeks significantly reduced fasting blood glucose levels, minimise testicular oxidative stress, increased testicular and epididymal catalase and epididymal SOD and increase total antioxidant activity capacity.
Treatment with vindoline showed protective effects against diabetes-induced oxidative stress in both testicular and epididymal tissues of male Wistar rats, hence can be considered potential agent in the management of diabetes-induced oxidative stress male sexual dysfunction.
本研究评估长春花碱对糖尿病诱导的雄性Wistar大鼠睾丸和附睾氧化应激的影响。
将48只6周龄、体重在190 - 230克之间的雄性Wistar大鼠分为6组（每组n = 8）用于本研究。第1组为正常对照组，第2组为用长春花碱治疗的非糖尿病大鼠组，第3组为用格列本脲（糖尿病治疗的标准药物）治疗的非糖尿病大鼠组。第4组为糖尿病对照组，第5组为用长春花碱治疗的糖尿病大鼠组，第6组为用格列本脲治疗的糖尿病大鼠组。对第4、5和6组大鼠，通过让它们饮用2周10%果糖水，随后单次腹腔注射40毫克/千克体重的链脲佐菌素（STZ）来诱导糖尿病。测定睾丸和附睾的脂质过氧化水平、抗氧化酶、清除活性和总抗氧化能力。
糖尿病诱导的雄性Wistar大鼠在睾丸和附睾组织中均表现出慢性高血糖、氧化应激和氧自由基吸收能力降低。用长春花碱对糖尿病大鼠进行5周的短期治疗可显著降低空腹血糖水平，使睾丸氧化应激最小化，增加睾丸和附睾的过氧化氢酶以及附睾的超氧化物歧化酶，并提高总抗氧化活性能力。
长春花碱治疗对雄性Wistar大鼠睾丸和附睾组织中糖尿病诱导的氧化应激具有保护作用，因此可被视为治疗糖尿病诱导的氧化应激所致男性性功能障碍的潜在药物。
