Datta Sudipto, Jana Shuvodeep, Das Ankita, Chakraborty Arindam, Chowdhury Amit Roy, Datta Pallab
Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah, 711103, WB, India.
Indian Institute of Technology, Kharagpur, West Bengal, India.
Bioact Mater. 2020 Apr 27;5(3):569-576. doi: 10.1016/j.bioactmat.2020.04.015. eCollection 2020 Sep.
Bioprinting has emerged as a potential technique to fabricate tissue engineering constructs and in vitro models directly using living cells as a raw material for fabrication, conforming to the heterogeneity and architectural complexity of the tissues. In several of tissue engineering and in vitro disease modelling or surgical planning applications, it is desirable to have radiopaque constructs for monitoring and evaluation. In the present work, enhanced radiopaque constructs are generated by substituting Calcium ions with Barium ions for crosslinking of alginate hydrogels. The constructs are characterized for their structural integrity and followed by cell culture studies to evaluate their biocompatibility. This was followed by the radiopacity evaluation. The radiological images obtained by micro-CT technique was further applied to investigate the degradation behavior of the scaffolds. In conclusion, it is observed that barium crosslinking can provide a convenient means to obtain radiopaque constructs with potential for multi-faceted applications.
生物打印已成为一种潜在技术，可直接使用活细胞作为制造原料来构建组织工程结构和体外模型，符合组织的异质性和结构复杂性。在组织工程、体外疾病建模或手术规划的多个应用中，需要有不透射线的结构用于监测和评估。在本研究中，通过用钡离子替代钙离子来交联藻酸盐水凝胶，生成了增强型不透射线结构。对这些结构进行结构完整性表征，随后进行细胞培养研究以评估其生物相容性。接着进行不透射线评估。通过微计算机断层扫描（micro-CT）技术获得的放射图像进一步用于研究支架的降解行为。总之，观察到钡交联可为获得具有多方面应用潜力的不透射线结构提供一种便捷方法。
