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嵌入式生物打印用于具有复杂结构组织的设计 3D 组织构建体。

Embedded bioprinting for designer 3D tissue constructs with complex structural organization.

机构信息

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China; NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an Jiaotong University, Xi'an, 710049, China.

State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, People's Republic of China; NMPA Key Lab for Research and Evaluation of Additive Manufacturing Medical Devices, Xi'an Jiaotong University, Xi'an, 710049, China.

出版信息

Acta Biomater. 2022 Mar 1;140:1-22. doi: 10.1016/j.actbio.2021.11.048. Epub 2021 Dec 5.

Abstract

3D bioprinting has been developed as an effective and powerful technique for the fabrication of living tissue constructs in a well-controlled manner. However, most existing 3D bioprinting strategies face substantial challenges in replicating delicate and intricate tissue-specific structural organizations using mechanically weak biomaterials such as hydrogels. Embedded bioprinting is an emerging bioprinting strategy that can directly fabricate complex structures derived from soft biomaterials within a supporting matrix, which shows great promise in printing large vascularized tissues and organs. Here, we provide a state-of-the-art review on the development of embedded bioprinting including extrusion-based and light-based processes to manufacture complex tissue constructs with biomimetic architectures. The working principles, bioinks, and supporting matrices of embedded printing processes are introduced. The effect of key processing parameters on the printing resolution, shape fidelity, and biological functions of the printed tissue constructs are discussed. Recent innovations in the processes and applications of embedded bioprinting are highlighted, such as light-based volumetric bioprinting and printing of functional vascularized organ constructs. Challenges and future perspectives with regard to translating embedded bioprinting into an effective strategy for the fabrication of functional biological constructs with biomimetic structural organizations are finally discussed. STATEMENT OF SIGNIFICANCE: It is still challenging to replicate delicate and intricate tissue-specific structural organizations using mechanically-weak hydrogels for the fabrication of functional living tissue constructs. Embedded bioprinting is an emerging 3D printing strategy that enables to produce complex tissue structures directly inside a reservoir filled with supporting matrix, which largely widens the choice of bioprinting inks to ECM-like hydrogels. Here we aim to provide a comprehensive review on various embedded bioprinting techniques mainly including extrusion-based and light-based processes. Various bioinks, supporting matrices, key processing parameters as well as their effects on the structures and biological functions of resultant living tissue constructs are discussed. We expect that it can provide an important reference and generate new insights for the bioprinting of large vascularized tissues and organs with biological functions.

摘要

3D 生物打印已发展成为一种有效且强大的技术,可用于以可控的方式制造活体组织构建体。然而,大多数现有的 3D 生物打印策略在使用机械强度较弱的生物材料(如水凝胶)复制精细而复杂的组织特异性结构组织方面面临着巨大的挑战。嵌入式生物打印是一种新兴的生物打印策略,它可以直接在支撑基质内制造源自软生物材料的复杂结构,在打印大型血管化组织和器官方面显示出巨大的潜力。在这里,我们提供了嵌入式生物打印技术的最新发展情况的综述,包括基于挤出和基于光的工艺,以制造具有仿生结构的复杂组织构建体。介绍了嵌入式打印工艺的工作原理、生物墨水和支撑基质。讨论了关键工艺参数对打印组织构建体的分辨率、形状保真度和生物学功能的影响。强调了嵌入式生物打印在工艺和应用方面的最新创新,例如基于光的体积生物打印和功能性血管化器官构建体的打印。最后,讨论了将嵌入式生物打印转化为制造具有仿生结构组织的功能性生物构建体的有效策略所面临的挑战和未来展望。

意义陈述

使用机械强度较弱的水凝胶来制造功能性活体组织构建体仍然难以复制精细而复杂的组织特异性结构组织。嵌入式生物打印是一种新兴的 3D 打印策略,它可以直接在充满支撑基质的储液器中产生复杂的组织结构,这大大拓宽了生物墨水的选择范围,使其可以使用类似于细胞外基质的水凝胶。在这里,我们旨在提供各种嵌入式生物打印技术的综合综述,主要包括基于挤出和基于光的工艺。讨论了各种生物墨水、支撑基质、关键工艺参数以及它们对所得活体组织构建体的结构和生物学功能的影响。我们希望这可以为具有生物功能的大型血管化组织和器官的生物打印提供重要参考并激发新的见解。

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