Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences/School of Basic Medicine, Peking Union Medical College, Beijing, China.
Key Laboratory of Endocrinology of Ministry of Health, Department of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
PLoS One. 2020 May 6;15(5):e0232084. doi: 10.1371/journal.pone.0232084. eCollection 2020.
Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.
关于小鼠棕色脂肪组织 (BAT) 蛋白质组的知识可以深入了解哺乳动物 BAT 的功能。在此，通过 2DLC 和高分辨率质谱对 C57BL/6J 雌性小鼠肩胛间 BAT 进行了全面分析，构建了小鼠 BAT 蛋白的综合蛋白质组数据集。共鉴定出 4949 个非冗余蛋白，并用 iBAQ 方法对 4495 个蛋白进行了定量。根据 iBAQ 值，BAT 蛋白质组被分为高丰度、中丰度和低丰度蛋白。高丰度蛋白的功能主要与葡萄糖和脂肪酸氧化以产生热量进行体温调节有关，而中丰度和低丰度蛋白的功能主要分别与蛋白质合成和细胞凋亡有关。此外，使用 SignalP4 软件预测 497 个蛋白具有信号肽，其中 75 个在先前的研究中得到证实。本研究首次全面描绘和功能注释了 BAT 蛋白质组。这项研究将有助于未来专注于生物标志物鉴定和 BAT 分子机制的研究。
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