COVID-2019 相关过表达普雷沃氏菌蛋白介导的宿主-病原体相互作用及其在冠状病毒爆发中的作用。
COVID-2019-associated overexpressed Prevotella proteins mediated host-pathogen interactions and their role in coronavirus outbreak.
机构信息
Division of Microbiology, Indian Council of Medical Research-National AIDS Research Institute, Pune, Maharashtra 411026, India.
Department of Pathology and Laboratory Medicine.
出版信息
Bioinformatics. 2020 Jul 1;36(13):4065-4069. doi: 10.1093/bioinformatics/btaa285.
MOTIVATION
The outbreak of COVID-2019 initiated at Wuhan, China has become a global threat by rapid transmission and severe fatalities. Recent studies have uncovered whole genome sequence of SARS-CoV-2 (causing COVID-2019). In addition, lung metagenomic studies on infected patients revealed overrepresented Prevotella spp. producing certain proteins in abundance. We performed host-pathogen protein-protein interaction analysis between SARS-CoV-2 and overrepresented Prevotella proteins with human proteome. We also performed functional overrepresentation analysis of interacting proteins to understand their role in COVID-2019 severity.
RESULTS
It was found that overexpressed Prevotella proteins can promote viral infection. As per the results, Prevotella proteins, but not viral proteins, are involved in multiple interactions with NF-kB, which is involved in increasing clinical severity of COVID-2019. Prevotella may have role in COVID-2019 outbreak and should be given importance for understanding disease mechanisms and improving treatment outcomes.
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
动机
在中国武汉爆发的 COVID-19 疫情通过快速传播和严重的致死率成为了全球性的威胁。最近的研究揭示了 SARS-CoV-2（引起 COVID-19 的病毒）的全基因组序列。此外，对感染患者的肺部宏基因组研究表明，某些产生特定蛋白质的普雷沃氏菌属过度表达。我们在 SARS-CoV-2 与过度表达的普雷沃氏菌蛋白和人类蛋白质组之间进行了宿主-病原体蛋白-蛋白相互作用分析。我们还对相互作用蛋白进行了功能过表达分析，以了解它们在 COVID-19 严重程度中的作用。
结果
研究发现过度表达的普雷沃氏菌蛋白可以促进病毒感染。根据结果，与 NF-kB 相互作用的不仅是病毒蛋白，还有普雷沃氏菌蛋白，NF-kB 参与了 COVID-2019 临床严重程度的增加。普雷沃氏菌可能在 COVID-2019 爆发中起作用，应该重视它在理解疾病机制和改善治疗结果方面的作用。
补充信息
补充数据可在“生物信息学在线”获得。
Zotero 插件