Xu Huachong, Li Haoxuan, Xu Junhao, Chen Yaoxin, Deng Li, Chen Xiaoyin, Xu Yinji
School of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China.
Zhongshan Hospital of Traditional Chinese Medicine, Zhongshan, Guangdong, China.
Front Med (Lausanne). 2025 Jul 7;12:1593007. doi: 10.3389/fmed.2025.1593007. eCollection 2025.
Most research reports on COVID-19 infections have focused on the correlation between the severity of the disease symptoms and immune deficits, while the mechanisms affecting the susceptibility to SARS-CoV-2 remain largely unknown. The study aimed to comprehensively analyze the differences in immunity, gut microbiota, metabolism, and proteomics between the SARS-CoV-2 resistant population and the susceptible population.
In this cohort comparison study, participants were rigorously selected based on inclusion and exclusion criteria in a continuous enrollment manner using combined questionnaires and clinical data, ultimately including 25 SARS-CoV-2 resistant volunteers versus 16 SARS-CoV-2 infected patients. The clinical information of the participants was recorded in detail, and fecal and blood samples were collected in a standardized manner for subsequent multi-omics analysis, including gut microbiota sequencing, metabolomics, and proteomics. This study has preliminarily elucidated the characteristics of the gut microbiota, serum metabolites, and serum proteins in the SARS-CoV-2 resistant population. It exhibits a unique metabolic signature characterized by elevated levels of serum phosphatidylinositol and the abundance of Prevotella, which may serve as a potential predictive biomarker for resistance to SARS-CoV-2.
Given the crucial role of phosphatidylinositol in cell membrane architecture and viral infectivity, this study provides a promising entry point for further research into the pathogenesis and prevention strategies of COVID-19.
大多数关于新冠病毒感染的研究报告都集中在疾病症状严重程度与免疫缺陷之间的相关性上，而影响对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）易感性的机制在很大程度上仍不清楚。本研究旨在全面分析SARS-CoV-2抗性人群和易感人群在免疫、肠道微生物群、代谢和蛋白质组学方面的差异。
在这项队列比较研究中，根据纳入和排除标准，采用联合问卷和临床数据以连续入组的方式严格筛选参与者，最终纳入25名SARS-CoV-2抗性志愿者和16名SARS-CoV-2感染患者。详细记录参与者的临床信息，并以标准化方式收集粪便和血液样本，用于后续的多组学分析，包括肠道微生物群测序、代谢组学和蛋白质组学。本研究初步阐明了SARS-CoV-2抗性人群的肠道微生物群、血清代谢物和血清蛋白的特征。它表现出一种独特的代谢特征，其特点是血清磷脂酰肌醇水平升高和普雷沃氏菌丰度增加，这可能作为对SARS-CoV-2抗性的潜在预测生物标志物。
鉴于磷脂酰肌醇在细胞膜结构和病毒感染性中的关键作用，本研究为进一步研究新冠病毒的发病机制和预防策略提供了一个有前景的切入点。
