Yang Dong Kwon, Lee Sei-Jin, Adam Gareeballah Osman, Kim Shang-Jin
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Jeonbuk National University, Iksan 54596, Korea.
Korea Basic Science Institute Jeonju Center, Jeonju 54896, Korea.
Antioxidants (Basel). 2020 May 4;9(5):379. doi: 10.3390/antiox9050379.
The present study aimed to evaluate the anti-fatigue effects of (AC) extract during exhaustive exercise of rats by forced swimming. Rats were subjected to forced swimming until exhausted after pre-treatment with AC extract for 21 days. Exhaustion time significantly increased in rats treated with AC extract. AC treatment also preserved blood homeostasis during fatigue due to exhaustive exercise. For fatigue-related serum biomarkers, AC extract significantly fail to decrease glucose and triglyceride (TG), but ameliorated increased lactate levels compared with levels in control rats. Metabolic acidosis, a major cause of fatigue, was effectively attenuated by AC extract, according to metabolic acidosis-related blood parameters. AC extract suppressed muscle injury and attenuated gastrocnemius muscle apoptotic responses due to exhaustive exercise. To investigate the mechanisms behind the AC extract anti-fatigue effect, we evaluated its effect on oxidative stress-related fatigue. We showed that pro-oxidants were inhibited, while antioxidants were preserved by AC extract treatment. Therefore, the anti-fatigue effect of AC extract was mediated by suppression of oxidative stress. Overall, the study demonstrated that AC extract effectively attenuates fatigue from exhaustive exercise through oxidative stress inhibition. AC extract, as an antioxidant, could be utilized as a therapeutic or preventive strategy against exhaustive exercise fatigue.
本研究旨在通过强迫游泳评估[提取物名称未给出]（AC）提取物在大鼠力竭运动期间的抗疲劳作用。在用AC提取物预处理21天后，使大鼠进行强迫游泳直至力竭。AC提取物处理的大鼠力竭时间显著延长。AC处理还在力竭运动引起的疲劳期间维持了血液稳态。对于与疲劳相关的血清生物标志物，AC提取物未能显著降低葡萄糖和甘油三酯（TG），但与对照大鼠相比，改善了升高的乳酸水平。根据与代谢性酸中毒相关的血液参数，AC提取物有效减轻了作为疲劳主要原因的代谢性酸中毒。AC提取物抑制了肌肉损伤，并减轻了因力竭运动导致的腓肠肌细胞凋亡反应。为了研究AC提取物抗疲劳作用背后的机制，我们评估了其对氧化应激相关疲劳的影响。我们发现AC提取物处理抑制了促氧化剂，同时保留了抗氧化剂。因此，AC提取物的抗疲劳作用是通过抑制氧化应激介导的。总体而言，该研究表明AC提取物通过抑制氧化应激有效减轻了力竭运动引起的疲劳。AC提取物作为一种抗氧化剂，可作为对抗力竭运动疲劳的治疗或预防策略。
