Laboratory of Marine Environmental Science, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.
Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Comp Biochem Physiol C Toxicol Pharmacol. 2020 Aug;234:108785. doi: 10.1016/j.cbpc.2020.108785. Epub 2020 May 3.
Pufferfish saxitoxin and tetrodotoxin binding proteins (PSTBPs) play an important role in the toxification of certain species of pufferfish. Recombinant Takifugu rubripes PSTBP1 (rTrub.PSTBP1) is reported to bind to tributyltin, and so it has been suggested that rTrub.PSTBP1 may reduce the toxicity of tributyltin. However, the role of PSTBP1 in vivo remains to be elucidated. Here, we established a transgenic medaka line showing whole-body Renilla reniformis green fluorescent protein and Trub.PSTBP1 expression, as confirmed by real-time polymerase chain reaction and mRNA-Seq analysis. mRNA-Seq analysis also showed that cytochrome P450 superfamily genes and the gene encoding ATP-binding cassette sub-family G member 2 were highly expressed in the transgenic medaka. Using embryos of the transgenic medaka line, we conducted an in ovo nanoinjection test to examine the effect of Trub.PSTBP1 in vivo, and obtained data suggesting that Trub.PSTBP1 expression may have reduced the toxicity of tributyltin in our transgenic medaka line. Our findings will be useful for future functional analyses of Trub.PSTBP1.
河豚毒素和鲀毒素结合蛋白（PSTBPs）在某些种类的河豚鱼的毒化过程中起着重要作用。据报道，重组赤点石斑鱼 PSTBP1（rTrub.PSTBP1）与三丁基锡结合，因此有人认为 rTrub.PSTBP1 可能降低三丁基锡的毒性。然而，PSTBP1 在体内的作用仍有待阐明。在这里，我们建立了一个转基因斑马鱼系，通过实时聚合酶链反应和 mRNA-Seq 分析证实了该系全身表达海肾绿色荧光蛋白和 Trub.PSTBP1。mRNA-Seq 分析还显示，细胞色素 P450 超家族基因和编码 ATP 结合盒亚家族 G 成员 2 的基因在转基因斑马鱼中高度表达。使用转基因斑马鱼系的胚胎，我们进行了体内纳米注射试验，以研究 Trub.PSTBP1 的体内作用，获得的数据表明，Trub.PSTBP1 的表达可能降低了我们的转基因斑马鱼系中三丁基锡的毒性。我们的发现将有助于未来对 Trub.PSTBP1 的功能分析。
