基于逆转录聚合酶链反应（RT-PCR）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）质谱法，对无毒养殖河豚（红鳍东方鲀）血浆中与河豚毒素和石房蛤毒素结合蛋白同源的亚型进行鉴定和区分。
RT-PCR- and MALDI-TOF mass spectrometry-based identification and discrimination of isoforms homologous to pufferfish saxitoxin- and tetrodotoxin-binding protein in the plasma of non-toxic cultured pufferfish (Takifugu rubripes).
作者信息
Tatsuno Ryohei, Yamaguchi Kenichi, Takatani Tomohiro, Arakawa Osamu
机构信息
Graduate School of Science and Technology, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, Japan.
出版信息
Biosci Biotechnol Biochem. 2013;77(1):208-12. doi: 10.1271/bbb.120701.
Four genes of Takifugu rubripes, tentatively designated Tr1-Tr4, encoding homologs of pufferfish saxitoxin- and tetrodotoxin-binding protein, were identified by BLAST search and 3'-RACE. RT-PCR and MALDI-TOF mass spectrometry allowed the identification and discrimination of Tr isoforms from the non-toxically cultured specimens. The expression of Tr1 and Tr3 mRNAs exclusively in the liver and the presence of their products as 120-kDa plasma proteins were confirmed.
通过BLAST搜索和3'-RACE技术，鉴定出红鳍东方鲀的四个基因，暂命名为Tr1-Tr4，它们编码河豚毒素和石房蛤毒素结合蛋白的同源物。逆转录聚合酶链反应（RT-PCR）和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF质谱）技术能够从无毒养殖标本中鉴定和区分Tr异构体。证实了Tr1和Tr3 mRNA仅在肝脏中表达，并且它们的产物以120 kDa血浆蛋白的形式存在。
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