de Souza Camilla Paulla, Alves Beatriz, Waisberg Jaques, Fonseca Fernando, Carmo Alipio de Oliveira, Gehrke Flavia
Departamento de Ciências da Saúde, Hospital do Servidor Público Estadual (Iamspe), São Paulo, São Paulo, Brazil.
Laboratório de Análises Clínicas, Centro Universitário Saúde ABC (CUS-ABC), Santo André, São Paulo, Brazil.
J Clin Pathol. 2020 Dec;73(12):826-829. doi: 10.1136/jclinpath-2020-206576. Epub 2020 May 6.
To determine the expression of the cyclooxygenase-2 () gene in patients with breast cancer attended at the Centro Universitário Saúde ABC/Faculdade de Medicina do ABC (CUS-ABC/FMABC) outpatient clinic. Breast cancer is the most common cancer in women worldwide. More than two million new cases are reported annually. An overexpression of has been observed in many cancers. is related to parameters of cancer aggressiveness, including tumour size, positive nodal state and lower survival, and to angiogenesis and resistance to apoptosis.
15 mL of peripheral blood was obtained from 34 patients and 21 healthy women. The extracellular RNA of QIAamp RNA was submitted to an RNA sequestration kit for RNA reverse transcriptase. Quantitative real-time PCR was performed using -specific oligonucleotides and the endogenous gene.
The mean remission time was 53 years. The mean progression time was 33 months. The difference observed between the patient and control groups in median expression (p<0.001) was significant.
Patients with breast cancer showed a higher mean expression in peripheral blood samples at diagnosis than the control group. Since this information could prove important in the diagnosis and prognosis of breast cancer, further research is required on larger patient samples.
确定在圣塔库鲁兹大学健康ABC分校/ABC医学院（CUS-ABC/FMABC）门诊就诊的乳腺癌患者中环氧合酶-2（）基因的表达情况。乳腺癌是全球女性中最常见的癌症。每年报告的新病例超过200万。在许多癌症中都观察到了的过表达。与癌症侵袭性参数有关，包括肿瘤大小、淋巴结阳性状态和较低的生存率，还与血管生成和抗凋亡有关。
从34例患者和21名健康女性中采集15毫升外周血。将QIAamp RNA的细胞外RNA提交给RNA逆转录酶的RNA螯合试剂盒。使用特异性寡核苷酸和内源性基因进行定量实时PCR。
平均缓解时间为53年。平均进展时间为33个月。患者组和对照组之间在中位数表达上的差异（p<0.001）具有显著性。
乳腺癌患者在诊断时外周血样本中的平均表达高于对照组。由于这些信息可能在乳腺癌的诊断和预后中具有重要意义，因此需要对更大的患者样本进行进一步研究。
