Pelstring R J, Swerdlow S H
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Ohio.
Mod Pathol. 1988 Jul;1(4):262-7.
Accurate and reproducible categorization of follicular center cells (FCC) on the basis of their nuclear size and shape is difficult. In addition, the relationship of cleaved to noncleaved FCC has been questioned. For these reasons, 2126 FCC (and 947 mantle cells) from five plastic embedded reactive lymph nodes were studied using a new morphometric approach. Using an image analyzer, the following nuclear features were studied: area (NA); our previously described shape factors which measure ellipticity (NCIe) and irregularity (NCIni); and a new objective measurement of relative chromatin dispersal (chromatin dispersal index, CDI). Definite clefts and nucleoli were visually identified and recorded. Compared to the mantle cells, FCC nuclei were significantly larger, more elliptical, more irregularly shaped, and had more dispersed chromatin. Among the FCC the only correlation that could be identified between the above parameters was that the larger FCC tended to have more dispersed chromatin (r = 0.46). Significantly more dispersed chromatin was, however, associated with nonclefted cells, less irregularly shaped cells having a NCIni less than the median NCIni, cells with nucleoli, and larger cells having a NA greater than the median NA. Clefted FCC had significantly greater nuclear irregularlity compared to nonclefted FCC but a similar degree of ellipticity. Distribution curves for the NA, NCIe, NCIni, and CDI revealed a continuous range of results rather than a limited number of distinct cell types. The addition of an objective quantitative measurement of chromatin dispersal permits a more complete morphometric description of FCC.(ABSTRACT TRUNCATED AT 250 WORDS)
基于核大小和形状对滤泡中心细胞（FCC）进行准确且可重复的分类很困难。此外，已裂解与未裂解FCC之间的关系也受到质疑。基于这些原因，我们使用一种新的形态测量方法对来自五个塑料包埋的反应性淋巴结的2126个FCC（以及947个套细胞）进行了研究。使用图像分析仪，研究了以下核特征：面积（NA）；我们之前描述的测量椭圆度（NCIe）和不规则度（NCIni）的形状因子；以及一种新的相对染色质分散度的客观测量方法（染色质分散指数，CDI）。通过视觉识别并记录明确的裂隙和核仁。与套细胞相比，FCC细胞核明显更大、更椭圆、形状更不规则，且染色质更分散。在FCC中，上述参数之间唯一可识别的相关性是较大的FCC往往具有更分散的染色质（r = 0.46）。然而，染色质更分散与未裂解细胞、NCIni小于中位数NCIni的形状较不规则细胞、有核仁的细胞以及NA大于中位数NA的较大细胞相关。与未裂解的FCC相比，裂解的FCC具有明显更大的核不规则度，但椭圆度程度相似。NA、NCIe、NCIni和CDI的分布曲线显示出连续的结果范围，而不是有限数量的不同细胞类型。增加染色质分散度的客观定量测量可以对FCC进行更完整的形态测量描述。（摘要截断于250字）
