Ekici Uğur, Ferhatoğlu Murat Ferhat, Çitgez Bülent, Uludağ Mehmet
Department of Health Sciences Collage, Istanbul Gelisim University, Istanbul, Turkey.
Department of General Surgery, Okan University Faculty of Medicine, Istanbul,Turkey.
Sisli Etfal Hastan Tip Bul. 2019 Jun 24;53(2):154-159. doi: 10.14744/SEMB.2019.98965. eCollection 2019.
Ankaferd BloodStopper (ABS) is a topical hemostatic agent that modulates the inflammatory response and accelerates wound healing. The aim of this study was to determine the effects of ABS on the colon anastomosis wound healing in a rat model.
Thirty-two Wistar albino rats were divided into four groups as follows: Group A (n=8), left colonic anastomosis plus ABS treatment (sacrificed on the 3 day); Group B (n=8) (control), left colonic anastomosis (sacrificed on the 3 day); Group C (n=8), left colonic anastomosis plus ABS treatment (sacrificed on the 7 day); and Group D (n=8) (control), left colonic anastomosis (sacrificed on the 7 day). All rats were sacrificed at the end of the experiment to assess the anastomosis integrity and the presence of perianastomosis abscesses, peritonitis, and adhesions. Additionally, the bursting pressure and hydroxyproline (OH-pyroline) levels were determined, and a histopathologic evaluation of the perianastomosis tissue was conducted.
The mean bursting pressure on Day 7 was significantly higher than that on Day 3 in the ABS group (p=0.017). Overall, the bursting pressure was higher in animals treated with ABS than in the control animals, although the difference was not statistically significant. The OH-pyroline levels of both ABS groups were significantly higher than in the control groups. The mean OH-pyroline level on Day 7 was higher than that on Day 3 in the ABS-treated animals (p=0.038).
ABS increases collagen formation and neovascularization, and it has a positive impact during the colon anastomosis healing in an experimental model of wound healing.
安卡非德止血剂（ABS）是一种局部止血剂，可调节炎症反应并加速伤口愈合。本研究的目的是确定ABS对大鼠模型结肠吻合口伤口愈合的影响。
将32只Wistar白化大鼠分为四组如下：A组（n = 8），左结肠吻合加ABS治疗（于第3天处死）；B组（n = 8）（对照组），左结肠吻合（于第3天处死）；C组（n = 8），左结肠吻合加ABS治疗（于第7天处死）；D组（n = 8）（对照组），左结肠吻合（于第7天处死）。在实验结束时处死所有大鼠，以评估吻合口完整性以及吻合口周围脓肿、腹膜炎和粘连的情况。此外，测定破裂压力和羟脯氨酸（OH-脯氨酸）水平，并对吻合口周围组织进行组织病理学评估。
ABS组第7天的平均破裂压力显著高于第3天（p = 0.017）。总体而言，接受ABS治疗的动物的破裂压力高于对照动物，尽管差异无统计学意义。两个ABS组的OH-脯氨酸水平均显著高于对照组。在接受ABS治疗的动物中，第7天的平均OH-脯氨酸水平高于第3天（p = 0.038）。
在伤口愈合的实验模型中，ABS可增加胶原蛋白形成和新血管形成，并对结肠吻合口愈合产生积极影响。
