Jeong Hwan-Jeong, Bagalkot Tarique Rajasaheb, Kim Hyeon Soo, Han Yeon-Hee, Kim Minjoo, Lim Seok Tae, Sohn Myung-Hee
Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Research Institute of Clinical Medicine of Jeonbuk National University and Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju, Jeonbuk Republic of Korea.
2Department of Cell biology, University of Pittsburgh, Pittsburgh, PA USA.
Nucl Med Mol Imaging. 2020 Apr;54(2):105-113. doi: 10.1007/s13139-020-00634-x. Epub 2020 Feb 22.
2-Deoxy-2-[F] fluoro-d-glucose positron emission tomography (F-FDG-PET) is a less-invasive and widely used diagnostic tool for detection of malignant tumors. However, prolonged retention of F-FDG in the body increases radiation exposure. This study evaluated the effect of oral administration of milk and ursodeoxycholic acid (UDCA) in terms of reducing radiation exposure by F-FDG.
F-FDG radioactivity was measured using a digital γ counter in the whole body and in various organs of rats after oral administration of milk and milk plus UDCA (milk + UDCA). Western blotting was performed to measure the expression levels of G6Pase, HK 2, CREB, FoxO1, and PGC-1α in the brain, liver, small intestine, and large intestine to assess the mechanism underlying the reduction in radiation exposure from F-FDG by oral administration of milk and UDCA.
We found a significant reduction in F-FDG radioactivity in the whole body and in the brain, liver, and small and large intestines. Expression of G6Pase was significantly increased in the above-mentioned organs in the milk and milk + UDCA groups. Expression of HK 2 was significantly decreased in the brain and small intestine in the milk and milk + UDCA groups. CREB, FoxO1, and PGC-1α expression levels in the brain, liver, and small intestine were increased in the milk and milk + UDCA groups. However, expression of PGC-1α in the large intestine in the milk and milk + UDCA groups was significantly decreased compared with that in the control group.
The present study demonstrated that administration of milk and UDCA increased G6Pase expression levels and F-FDG release from the tissue. These results suggest milk and UDCA could be used to reduce radiation exposure from F-FDG after image acquisition. The mechanisms underpinning this phenomenon should be explored in a human study.
2-脱氧-2-[F]氟-D-葡萄糖正电子发射断层扫描（F-FDG-PET）是一种用于检测恶性肿瘤的侵入性较小且广泛使用的诊断工具。然而，F-FDG在体内的长时间滞留会增加辐射暴露。本研究评估了口服牛奶和熊去氧胆酸（UDCA）在减少F-FDG辐射暴露方面的效果。
在大鼠口服牛奶以及牛奶加UDCA（牛奶+UDCA）后，使用数字γ计数器测量全身及各个器官中的F-FDG放射性。进行蛋白质免疫印迹法以测量大脑、肝脏、小肠和大肠中葡萄糖-6-磷酸酶（G6Pase）、己糖激酶2（HK 2）、环磷腺苷效应元件结合蛋白（CREB）、叉头框蛋白O1（FoxO1）和过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）的表达水平，以评估口服牛奶和UDCA减少F-FDG辐射暴露的潜在机制。
我们发现全身以及大脑、肝脏、小肠和大肠中的F-FDG放射性显著降低。牛奶组和牛奶+UDCA组上述器官中G6Pase的表达显著增加。牛奶组和牛奶+UDCA组大脑和小肠中HK 2的表达显著降低。牛奶组和牛奶+UDCA组大脑、肝脏和小肠中CREB、FoxO1和PGC-1α的表达水平升高。然而，与对照组相比，牛奶组和牛奶+UDCA组大肠中PGC-1α的表达显著降低。
本研究表明，给予牛奶和UDCA可提高G6Pase表达水平并促进组织中F-FDG的释放。这些结果表明，牛奶和UDCA可用于在图像采集后减少F-FDG的辐射暴露。这一现象背后的机制应在人体研究中进行探索。
