荧光素单葡萄糖醛酸苷的体外合成。
In vitro synthesis of fluorescein monoglucuronide.
作者信息
Guenthner T M, Blair N P
机构信息
Department of Pharmacology, University of Illinois College of Medicine, Chicago.
出版信息
Pharmacology. 1988;37(5):341-8. doi: 10.1159/000138486.
PMID:3237763
Abstract
A simple, economical method for the in vitro synthesis of milligram amounts of fluorescein monoglucuronide is presented. The compound can be synthesized in 1 day, and 2 chromatographic steps result in complete purification. The synthetic fluorescein monoglucuronide was found to be identical to biosynthetic fluorescein monoglucuronide by spectrophotometric and fluorometric criteria.
摘要
本文介绍了一种体外合成毫克量荧光素单葡萄糖醛酸苷的简单、经济的方法。该化合物可在1天内合成，经过两步色谱法可实现完全纯化。通过分光光度法和荧光分析法，发现合成的荧光素单葡萄糖醛酸苷与生物合成的荧光素单葡萄糖醛酸苷相同。
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