HERCULES Lab., Departamento de Química, Escola de Ciências e Tecnologia, Universidade de Évora, 7000-809 Évora, Portugal.
CEB-Biological Engineering Centre, Micoteca da Universidade do Minho, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Int J Syst Evol Microbiol. 2020 May;70(5):3475-3482. doi: 10.1099/ijsem.0.004201.
This study describes a novel fungal species belonging to the genus . During a previous work focusing on metalaxyl degradation by strains, two isolates from vineyard soil samples collected in the Alentejo region, south Portugal, were identified as a putative novel species based on combined molecular and MALDI-TOF MS data. This new species is described here using a polyphasic approach that combines morphology, internal transcribed spacer of ribosomal DNA (ITS) and 28S ribosomal DNA (LSU) sequence data analysis and proteomic profiling by MALDI-TOF MS. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other species with results of combined ITS+LSU analysis showing that the species is related to and . Therefore, from the results of morphological and molecular analyses, isolates MUM 10.262 and MUM 10.263 seem to represent a new species and the name sp. nov. is proposed, with the ex-type strain MUM 10.262 (=CCMI 1100=CBS 128763).
本研究描述了一个属于 属的新型真菌物种。在之前的一项专注于金属axyl 降解的研究中，从葡萄牙南部阿尔加维地区的葡萄园土壤样本中分离出的两个菌株，根据联合的分子和 MALDI-TOF MS 数据被鉴定为一个假定的新型物种。本文采用多相方法描述了这个新物种，该方法结合了形态学、核糖体 DNA 的内部转录间隔区（ITS）和 28S 核糖体 DNA（LSU）序列数据分析以及 MALDI-TOF MS 的蛋白质组分析。表型和分子数据使该新型物种能够与其他 物种清楚地区分，ITS+LSU 联合分析的结果表明，该物种与 和 有关。因此，从形态学和分子分析的结果来看，MUM 10.262 和 MUM 10.263 似乎代表了一个新的 物种，因此提出了 sp. nov. 的名称，其模式菌株为 MUM 10.262（=CCMI 1100=CBS 128763）。
