A Reversibly Induced CRISPRi System Targeting Photosystem II in the Cyanobacterium sp. PCC 6803.

The cyanobacterium sp. PCC 6803 is used as a model organism to study photosynthesis, as it can utilize glucose as the sole carbon source to support its growth under heterotrophic conditions. CRISPR interference (CRISPRi) has been widely applied to repress the transcription of genes in a targeted manner in cyanobacteria. However, a robust and reversible induced CRISPRi system has not been explored in 6803 to knock down and recover the expression of a targeted gene. In this study, we built a tightly controlled chimeric promoter, P, in which a theophylline responsive riboswitch was integrated into a rhamnose-inducible promoter system. We applied this promoter to drive the expression of ddCpf1 (DNase-dead Cpf1 nuclease) in a CRISPRi system and chose the PSII reaction center gene (D2 protein) to target for repression. was specifically knocked down by over 95% of its native expression, leading to severely inhibited photosystem II activity and growth of 6803 under photoautotrophic conditions. Significantly, removal of the inducers rhamnose and theophylline reversed repression by CRISPRi. Expression of PsbD recovered following release of repression, coupled with increased photosystem II content and activity. This reversibly induced CRISPRi system in 6803 represents a new strategy for study of the biogenesis of photosynthetic complexes in cyanobacteria.