College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China; State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China.
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, PR China.
Bioresour Technol. 2020 Aug;309:123378. doi: 10.1016/j.biortech.2020.123378. Epub 2020 Apr 15.
Biological pretreatment is a promising technology to increase biogas yield. The methane yield and microbial community resulting from anaerobic digestion of maize straw after pretreatment of enzymes [extracted from Trichoderma viride (ETv) and Aspergillus sp. (EAs)] at different mixing ratios [5/0, 4/1, 3/2, 2/3, 1/4, 0/5] were evaluated. The methane yields from mixed enzymes pretreatment were higher than single enzymes pretreatments of ETv and EAs. The optimal enzymes mixing ratio of ETv and EAs was found to be 2/3, with the cumulative methane yield 512.64 mL/g TS, which was 31.74% higher than the control. Enzymatic pretreatment promoted an increase in the abundance of bacteria and archaea associated with cellulose decomposition. The majority of bacteria and archaea were assigned to Bacteroidetes, Firmicutes and Methanosaeta.
生物预处理是一种提高沼气产量的有前途的技术。通过在不同混合比例[5/0、4/1、3/2、2/3、1/4、0/5]下用酶[从绿色木霉（ETv）和曲霉属（EAs）中提取]对玉米秸秆进行预处理，评估了预处理后厌氧消化的甲烷产量和微生物群落。混合酶预处理的甲烷产量高于 ETv 和 EAs 的单一酶预处理。发现 ETv 和 EAs 的最佳酶混合比例为 2/3，累积甲烷产量为 512.64 mL/g TS，比对照提高了 31.74%。酶预处理促进了与纤维素分解相关的细菌和古菌丰度的增加。大多数细菌和古菌被归为拟杆菌门、厚壁菌门和产甲烷菌门。
