School of Pharmacy, Changzhou University, Changzhou, 213164, Jiangsu, China.
Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, Jiangsu, China.
Mikrochim Acta. 2023 Aug 19;190(9):357. doi: 10.1007/s00604-023-05926-5.
Novel chiral capillary electrochromatography (CEC) microsystems were constructed based on Aspergillus sp. CM96. As a newly discovered intrinsic characteristic of the cell, cell chirality occupies an essential position in life evolution. Aspergillus sp. CM96 spore (CM96s) was chosen as a proof of concept to develop chiral capillary columns. Interestingly, various types of amino acid (AA) enantiomers were baseline separated under the optimized conditions. Furthermore, the time-dependent chiral interactions between AAs and CM96s were explored in a wider space. Pectinases generated from Aspergillus sp. CM96 fermentation were immobilized onto graphene oxide-functionalized capillary silica monoliths for separating AA enantiomers. Molecular docking simulations were performed to explore chiral separation mechanisms of pectinase for AA enantiomers. These results indicated that Aspergillus sp. CM96-based CEC microsystems have a significant advantage for chiral separation.
基于青霉 CM96 构建了新型手性毛细管电色谱（CEC）微系统。细胞手性作为细胞的一种新发现的内在特性，在手性拆分中占据着重要的地位。青霉 CM96 孢子（CM96s）被选为概念验证，以开发手性毛细管柱。有趣的是，在优化条件下，各种类型的氨基酸（AA）对映体得到基线分离。此外，还在更广泛的空间内探索了 AA 与 CM96s 之间的时间依赖性手性相互作用。从青霉 CM96 发酵中产生的果胶酶被固定到氧化石墨烯功能化的毛细管硅胶整体柱上，用于分离 AA 对映体。进行分子对接模拟以探索果胶酶对 AA 对映体的手性分离机制。这些结果表明，基于青霉 CM96 的 CEC 微系统在手性分离方面具有显著优势。
