Herrero M V, Odell G V
Facultad de Ciencias Exactas y Naturales, Universidad Nacional, San José, Costa Rica.
Rev Biol Trop. 1988 Nov;36(2A):241-6.
A comparison of some components of the venoms of two Costa Rican tarantulas, Aphonopelma seemanni (Cambridge) and Sphaerobothria hoffmanni (Karsch) by polyacrylamide gel electrophoresis shows patterns similar to those of Dugesiella hentzi (Girard), a North American tarantula. The digestive secretions have proteins that do not enter the 15% gels; thus no bands are observed. The method used by the tarantulas to consume their prey involves the action of both the venom and the digestive secretions. The percent protein, pH, proteolytic activity and hemolytic activity of venom and digestive secretions of both species were determined, and a high proteolytic activity for digestive secretions was found.
通过聚丙烯酰胺凝胶电泳对两种哥斯达黎加狼蛛——塞曼尼食鸟蛛(剑桥)和霍夫曼球蛛(卡尔斯)毒液的某些成分进行比较,结果显示其图谱与北美狼蛛亨氏杜盖蛛(吉拉德)的图谱相似。消化分泌物中的蛋白质无法进入15%的凝胶;因此未观察到条带。狼蛛捕食猎物所采用的方法涉及毒液和消化分泌物的共同作用。测定了这两种狼蛛毒液和消化分泌物的蛋白质百分比、pH值、蛋白水解活性和溶血活性,发现消化分泌物具有较高的蛋白水解活性。